人嗜酸性粒细胞的分离
Isolation of human eosinophils.
作者信息
Akuthota Praveen, Shamri Revital, Weller Peter F
机构信息
Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
出版信息
Curr Protoc Immunol. 2012 Aug;Chapter 7:7.31.1-7.31.8. doi: 10.1002/0471142735.im0731s98.
Abstract
Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and differentiation from other leukocyte populations.
摘要
通过基于抗体的磁性阴性选择方案进行阴性选择，可以从外周血中分离出高度纯化的嗜酸性粒细胞。基本方案描述了外周血的连续分级分离，其中首先从全血中富集CD16+粒细胞，然后分离嗜酸性粒细胞。该技术易于使用、快速且具有高度可重复性。支持方案描述了可用于评估嗜酸性粒细胞纯度以及与其他白细胞群体区分的染色方法。
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