用于动态细胞黏附研究的环形、纳米结构和生物功能化水凝胶微通道。
Circular, nanostructured and biofunctionalized hydrogel microchannels for dynamic cell adhesion studies.
机构信息
Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr, Stuttgart, 370569, Germany.
出版信息
Lab Chip. 2012 Sep 21;12(18):3285-9. doi: 10.1039/c2lc40611j. Epub 2012 Aug 2.
Abstract
We report on a method to fabricate biofunctionalized polyethylene glycol hydrogel microchannels with adjustable circular cross-sections. The inner channel surfaces are decorated with Au-nanoparticle arrays of tunable density. These Au-nanoparticles are functionalized with biomolecules whereas the hydrogel material provides an inert and biocompatible background. This technology provides control over flow conditions, channel curvature and biomolecule density on the channel surface. It can be applied for biophysical studies of cell-surface interactions mimicking, for example, leukocyte interactions with the endothelial lining in small vessels.
摘要
我们报告了一种制造具有可调圆形横截面的生物功能化聚乙二醇水凝胶微通道的方法。内通道表面装饰有可调密度的金纳米粒子阵列。这些金纳米粒子通过生物分子功能化，而水凝胶材料提供惰性和生物相容性的背景。该技术可控制流条件、通道曲率和通道表面上的生物分子密度。它可用于模拟细胞表面相互作用的生物物理研究，例如白细胞与小血管内皮衬里的相互作用。
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