TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands.
Alcohol Alcohol. 2013 Mar-Apr;48(2):153-9. doi: 10.1093/alcalc/ags086. Epub 2012 Aug 2.
To evaluate the effect of acute and chronic consumption of red wine or de-alcoholized red wine with a similar antioxidant capacity on plasma total antioxidant capacity (TEAC), nuclear factor-κB (NF-κB) activity and F2-isoprostanes (8-iso-PGF(2α)) in healthy men.
Nineteen healthy men with an increased waist circumference (≥94 cm) and a body mass index above 25 kg/m(2) participated in a randomized, controlled crossover design trial. They daily consumed 450 ml of red wine (four drinks; 41.4 g alcohol) or 450 ml of de-alcoholized red wine during dinner for 4 weeks each. On the last day of each treatment period, blood was collected before and 1 h after a standardized dinner with red wine or de-alcoholized red wine and also 24-h urine was collected.
Absolute TEAC levels were higher 1 h after dinner with red wine compared with dinner with de-alcoholized red wine (1.3 versus 1.1 mmol Trolox equivalents/l; P = 0.03). Consumption of dinner together with de-alcoholized red wine acutely stimulated NF-κB activity in peripheral blood mononuclear cells (0.4-0.7 HeLa equivalents/2.5 μg protein; P = 0.006), whereas this increase was completely suppressed when the dinner was combined with red wine. A chronic increase in urinary 8-iso-PGF(2α) after 4 weeks of red wine consumption compared with de-alcoholized red wine consumption (157 pg/mg creatinine and 141 pg/mg creatinine, respectively, P = 0.006) was also observed.
Consumption of a moderate dose of red wine can acutely increase plasma TEAC and suppress NF-κB activation induced by a meal. Controversially, 4 weeks of red wine consumption compared with de-alcoholized red wine consumption increases the oxidative lipid damage marker 8-iso-PGF(2α).
评估急性和慢性饮用红葡萄酒或具有相似抗氧化能力的去醇红葡萄酒对健康男性血浆总抗氧化能力（TEAC）、核因子-κB（NF-κB）活性和 F2-异前列腺素（8-iso-PGF（2α））的影响。
19 名腰围增加（≥94cm）和体重指数超过 25kg/m（2）的健康男性参加了一项随机、对照交叉设计试验。他们每天在晚餐时饮用 450ml 红葡萄酒（四杯；41.4g 酒精）或 450ml 去醇红葡萄酒，每种治疗持续 4 周。在每个治疗期的最后一天，在晚餐时饮用红葡萄酒或去醇红葡萄酒前后 1 小时采集血液，同时采集 24 小时尿液。
与晚餐时饮用去醇红葡萄酒相比，红葡萄酒餐后 1 小时的绝对 TEAC 水平更高（1.3 与 1.1mmol Trolox 当量/L；P=0.03）。与饮用去醇红葡萄酒的晚餐相比，饮用含酒精的晚餐会急性刺激外周血单核细胞中的 NF-κB 活性（0.4-0.7 HeLa 当量/2.5μg 蛋白；P=0.006），而当晚餐与红葡萄酒一起饮用时，这种增加则完全被抑制。与饮用去醇红葡萄酒相比，饮用红葡萄酒 4 周后尿液中 8-iso-PGF（2α）的慢性增加（分别为 157pg/mg 肌酐和 141pg/mg 肌酐，P=0.006）也观察到。
饮用适量的红葡萄酒可急性增加血浆 TEAC 并抑制膳食引起的 NF-κB 激活。相反，与去醇红葡萄酒相比，饮用红葡萄酒 4 周会增加氧化脂质损伤标志物 8-iso-PGF（2α）。
