类咕啉和类卟啉生物合成的研究。III. 维生素B12酰胺氮的来源。

Studies on the biosynthesis of corrinoids and porphyrinoids. III. The origin of amide nitrogen of vitamin B12.

作者信息

Kurumaya K, Kajiwara M

机构信息

Department of Medicinal Chemistry, Meiji College of Pharmacy, Tokyo, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1990 Sep;38(9):2589-90. doi: 10.1248/cpb.38.2589.

DOI:10.1248/cpb.38.2589

PMID:2285991

Abstract

To clarify the origin of amide-nitrogen of vitamin B12, [1-13C]aminolevulinic acid (ALA) and L-[amide-15N]glutamine were administered to P. shermanii. The 13C-nuclear magnetic resonance spectrum of the vitamin B12 subsequently isolated showed distinct 13C-15N coupling and isotope shift at six amide carbons. However, the C-57 amide carbon showed neither coupling, nor shift. Thus, it was concluded that the nitrogens of 6 amides of the side chain were derived from glutamine and the C-57 amide nitrogen was from threonine.

摘要

为阐明维生素B12酰胺氮的来源，将[1-13C]氨基乙酰丙酸（ALA）和L-[酰胺-15N]谷氨酰胺给予谢氏丙酸杆菌。随后分离得到的维生素B12的13C核磁共振谱显示，在六个酰胺碳处有明显的13C-15N偶合和同位素位移。然而，C-57酰胺碳既没有偶合也没有位移。因此，得出结论，侧链6个酰胺的氮来自谷氨酰胺，C-57酰胺氮来自苏氨酸。