Kurumaya K, Okazaki T, Kajiwara M
Department of Medicinal Chemistry, Meiji College of Pharmacy, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 1990 Apr;38(4):1058-61. doi: 10.1248/cpb.38.1058.
To clarify the origin of nitrogen of vitamin B12, 15N-labeled aminolevulinic acid (ALA) was prepared and administered to Propionibacterium shermanii. Vitamin B12 thus isolated showed four signals in the nitrogen-15 nuclear magnetic resonance (15N-NMR) spectrum. The nitrogen of [5-15N]riboflavine was incorporated into the benzimidazole part of vitamin B12. Hydroxycobalamin was transformed into cyanocobalamin by treatment with [15N]potassium cyanide, and the 15N-NMR spectrum was measured. The results of these experiments revealed the origin of the nitrogen atoms of vitamin B12, and allowed the 15N-NMR signals to be assigned.
为阐明维生素B12中氮的来源，制备了15N标记的氨基乙酰丙酸（ALA）并将其给予谢氏丙酸杆菌。由此分离得到的维生素B12在氮-15核磁共振（15N-NMR）谱中显示出四个信号。[5-15N]核黄素中的氮被并入维生素B12的苯并咪唑部分。通过用[15N]氰化钾处理，将羟基钴胺素转化为氰钴胺素，并测量了15N-NMR谱。这些实验结果揭示了维生素B12中氮原子的来源，并确定了15N-NMR信号。
