维生素B12的生物合成：关于咕啉核次甲基质子的起源
Biosynthesis of vitamin B12: concerning the origin of the methine protons of the corrin nucleus.
作者信息
Scott A I, Kajiwara M, Santander P J
机构信息
Department of Chemistry, Texas A&M University, College Station 77843.
出版信息
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6616-8. doi: 10.1073/pnas.84.19.6616.
Abstract
13C NMR spectroscopy has been used to locate six deuterium atoms incorporated biosynthetically on the periphery of the corrin nucleus of vitamin B12 (cyanocobalamin) derived from cells of Propionibacterium shermanii grown in a medium containing 50% 2H2O and 13C-enriched delta-aminolevulinic acid. The implications of these results for the mechanism of vitamin B12 biosynthesis are discussed, and it is concluded that the same oxidation level of the intermediates is maintained throughout the biosynthetic pathway, from delta-aminolevulinic acid to corrin.
摘要
13C核磁共振光谱已被用于确定六个通过生物合成掺入维生素B12（氰钴胺素）钴啉核外围的氘原子，该维生素B12来源于在含有50% 2H2O和13C富集的δ-氨基乙酰丙酸的培养基中生长的谢氏丙酸杆菌细胞。讨论了这些结果对维生素B12生物合成机制的影响，并得出结论，从δ-氨基乙酰丙酸到钴啉，整个生物合成途径中中间体的氧化水平保持不变。
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