基于 BODIPY 的分子探针用于脑β-淀粉样斑块成像。
BODIPY-based molecular probe for imaging of cerebral β-amyloid plaques.
机构信息
Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University , 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
出版信息
ACS Chem Neurosci. 2012 Apr 18;3(4):319-24. doi: 10.1021/cn3000058. Epub 2012 Feb 17.
Abstract
We designed and synthesized a BODIPY-based probe (BAP-1) for the imaging of β-amyloid plaques in the brain. In binding experiments in vitro, BAP-1 showed excellent affinity for synthetic Aβ aggregates. β-Amyloid plaques in Tg2576 mouse brain were clearly visualized with BAP-1. In addition, the labeling of β-amyloid plaques was demonstrated in vivo in Tg2576 mice. These results suggest BAP-1 to be a useful fluorescent probe for the optical imaging of cerebral β-amyloid plaques in patients with Alzheimer's disease.
摘要
我们设计并合成了一种基于 BODIPY 的探针（BAP-1），用于大脑中β-淀粉样斑块的成像。在体外结合实验中，BAP-1 显示出对合成 Aβ 聚集体的优异亲和力。BAP-1 可清晰地观察到 Tg2576 小鼠脑内的β-淀粉样斑块。此外，还在 Tg2576 小鼠体内证明了β-淀粉样斑块的标记。这些结果表明 BAP-1 是一种有用的荧光探针，可用于光学成像阿尔茨海默病患者大脑中的β-淀粉样斑块。
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