Departamento de Bioquímica e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia da Fixação Biológica de Nitrogênio, UFPR Curitiba, PR, Brazil.
FEMS Microbiol Rev. 2013 Mar;37(2):251-83. doi: 10.1111/j.1574-6976.2012.00351.x. Epub 2012 Aug 28.
The P(II) proteins are one of the most widely distributed families of signal transduction proteins in nature. They are pivotal players in the control of nitrogen metabolism in bacteria and archaea, and are also found in the plastids of plants. Quite remarkably, P(II) proteins control the activities of a diverse range of enzymes, transcription factors and membrane transport proteins, and in recent years the extent of these interactions has been recognized to be much greater than heretofore described. Major advances have been made in structural studies of P(II) proteins, including the solution of the first structures of P(II) proteins complexed with their targets. We have also begun to gain insights into how the key effector molecules, 2-oxoglutarate and ATP/ADP, influence the activities of P(II) proteins. In this review, we have set out to summarize our current understanding of P(II) biology and to consider where future studies of these extraordinarily adaptable proteins might lead us.
P(II) 蛋白是自然界中分布最广泛的信号转导蛋白家族之一。它们在细菌和古菌氮代谢的控制中起着关键作用，也存在于植物的质体中。非常值得注意的是，P(II) 蛋白控制着多种酶、转录因子和膜转运蛋白的活性，近年来，人们认识到这些相互作用的程度比以前描述的要大得多。在 P(II) 蛋白的结构研究方面取得了重大进展，包括首次解决了与靶标结合的 P(II) 蛋白的结构。我们也开始深入了解关键效应分子 2-氧戊二酸和 ATP/ADP 如何影响 P(II) 蛋白的活性。在这篇综述中，我们旨在总结我们目前对 P(II) 生物学的理解，并考虑未来对这些适应性极强的蛋白质的研究可能会带来哪些启示。
