Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Ibadan, Nigeria.
Int J Tuberc Lung Dis. 2012 Oct;16(10):1388-92. doi: 10.5588/ijtld.12.0109. Epub 2012 Aug 3.
Bovine tuberculosis (TB) is endemic in the cattle population in Nigeria. Livestock workers are at risk of Mycobacterium bovis infection and unaware of their health status.
To determine the occurrence of pulmonary M. bovis infection among livestock workers.
A cross-sectional study of livestock traders was conducted for TB through screening of sputum samples using a simple random sampling method coupled with oral interview on the assumption of sub-clinical pulmonary TB infection. Specimens were cultured, and the isolates analysed using molecular typing techniques.
Overall, 10% (7/70) of the livestock traders had a positive culture indicative of M. tuberculosis complex, which were differentiated into M. bovis (n = 2) and M. tuberculosis (n = 5) using deletion typing. Further spoligotyping analyses of the M. bovis and two available M. tuberculosis isolates classified the strains as SB1432 and SB09444 and LAM_10 CAM and T1 using respectively www.mbovis.org and spotclust databases. Prolonged cough and >3 years in the livestock trade were risk factors for infection.
We confirm that there is undetected pulmonary M. bovis infection among livestock traders in Nigeria. Further studies on the role of occupationally exposed workers in the transmission of M. bovis infection to the larger community are required.
在尼日利亚，牛结核病（TB）在牛群中流行。牲畜工作者面临感染牛分枝杆菌的风险，但他们可能并不知道自己的健康状况。
确定牲畜工作者中是否存在肺部 M. bovis 感染。
采用简单随机抽样方法对牲畜交易员进行了一项横断面研究，对疑似潜伏性肺结核感染的痰液样本进行了结核病筛查，并通过口腔访谈进行了检测。采集标本进行培养，并采用分子分型技术对分离株进行分析。
总体而言，70 名牲畜交易员中有 10%（7/70）的痰液培养呈阳性，提示存在结核分枝杆菌复合群，其中 2 株为牛分枝杆菌，5 株为结核分枝杆菌。进一步采用缺失型分型技术对 2 株牛分枝杆菌和 2 株可获得的结核分枝杆菌分离株进行 spoligotyping 分析，结果显示这些菌株分别为 SB1432 和 SB09444 以及 LAM_10 CAM 和 T1，分别使用了 www.mbovis.org 和 spotclust 数据库。长期咳嗽和从事牲畜交易超过 3 年是感染的危险因素。
我们证实，在尼日利亚的牲畜交易员中存在未被发现的肺部 M. bovis 感染。需要进一步研究职业接触者在牛分枝杆菌感染向更大社区传播中的作用。
