Institute of Microbiology and Infection, University of Birmingham, Birmingham B15 2TT, UK.
Nat Rev Microbiol. 2012 Sep;10(9):599-606. doi: 10.1038/nrmicro2850. Epub 2012 Aug 6.
Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.
在这里，我们对 2012 年微生物学家可利用的高通量测序平台及其相关分析工具进行了概述，并评估了这些平台在获取细菌基因组序列方面的优缺点。我们还展望了未来的可能性，推测测序成本“低到无法计量”的可用性将如何永远改变微生物学的面貌。
