Civzele Anna, Mezule Linda
Water Systems and Biotechnology Institute, Faculty of Natural Sciences and Technology, Riga Technical University, Riga, Latvia.
Front Fungal Biol. 2024 Dec 19;5:1494182. doi: 10.3389/ffunb.2024.1494182. eCollection 2024.
The growing demand for novel enzyme producers to meet industrial and environmental needs has driven interest in lignocellulose-degrading fungi. In this study, lignocellulolytic enzyme production capabilities of environmental fungal isolates collected from boreal coniferous and nemoral summer green deciduous forests were investigated, using Congo Red, ABTS, and Azure B as indicators of cellulolytic and ligninolytic enzyme productions. Through qualitative and quantitative assays, the study aimed to identify promising species for lignocellulose-degrading enzyme secretion and assess their potential for biotechnological applications. Primary screening tests showed intensive enzyme secretion by certain isolates, particularly white rot fungi identified as and . These fungi exhibited high efficiency in degrading Congo Red and Azure B. The isolates achieved up to a 93.30% decrease in Congo Red induced color intensity and over 78% decolorization of Azure B within 168 hours. Within 336 hours, these fungi reached nearly 99% removal of Congo Red and up to 99.79% decolorization of Azure B. Enzyme activity analysis confirmed the lignin-degrading capabilities of , which exhibited laccase activity exceeding 208 U/mL. Furthermore, showed the highest cellulose-degrading potential among the studied fungi, achieving cellulase activity over 107 U/L during Congo Red decolorization. Previously undescribed enzyme-producing species, such as , , and , also demonstrated promising lignocellulolytic enzyme production potential, achieving up to 98.65% and 99.80% decolorization of Congo Red and Azure B, respectively. The study demonstrates novel candidates for efficient lignocellulolytic enzyme production with broad biotechnological applications such as biomass conversion, wastewater treatment, textile dye and other complex chemical removal, and environmental remediation.
为满足工业和环境需求，对新型酶生产者的需求不断增长，这激发了人们对木质纤维素降解真菌的兴趣。在本研究中，使用刚果红、ABTS和天青B作为纤维素分解酶和木质素分解酶产生的指标，对从北方针叶林和温带夏绿阔叶林采集的环境真菌分离株的木质纤维素分解酶生产能力进行了研究。通过定性和定量分析，该研究旨在确定有前景的木质纤维素降解酶分泌物种，并评估它们在生物技术应用中的潜力。初步筛选试验表明，某些分离株，特别是被鉴定为 和 的白腐真菌，能大量分泌酶。这些真菌在降解刚果红和天青B方面表现出高效率。在168小时内，这些分离株使刚果红诱导的颜色强度降低了93.30%，天青B的脱色率超过78%。在336小时内，这些真菌使刚果红的去除率接近99%，天青B的脱色率高达99.79%。酶活性分析证实了 的木质素降解能力，其漆酶活性超过208 U/mL。此外， 在研究的真菌中表现出最高的纤维素降解潜力，在刚果红脱色过程中纤维素酶活性超过107 U/L。以前未描述的产酶物种，如 、 和 ，也显示出有前景的木质纤维素分解酶生产潜力，刚果红和天青B的脱色率分别高达98.65%和99.80%。该研究证明了高效木质纤维素分解酶生产的新候选物种，具有广泛的生物技术应用，如生物质转化、废水处理、纺织染料和其他复杂化学物质的去除以及环境修复。
