Xia Xiao-Ming, Jin Wei-Yun, Shi Ren-Zhong, Zhang Ya-Feng, Chen Jun
Department of Thoracic Surgery, Jinshan Branch of the Sixth People's Hospital, Affiliated with Shanghai Jiaotong University, Shanghai 201500, P.R. China.
Oncol Lett. 2010 Nov;1(6):1045-1047. doi: 10.3892/ol.2010.164. Epub 2010 Sep 1.
To detect the expression levels of the lethal-7 (let-7) and K-ras genes in non-small cell lung cancer (NSCLC) and investigate their significance, the expression of let-7 and K-ras was determined in cancerous tissues and pericancerous normal tissues of 31 NSCLC patients using reverse transcription-polymerase chain reaction. Our results using 31 NSCLC patient samples showed that 67.74% had a low let-7 expression (P<0.05), 64.52% had a high K-ras expression (P<0.05), and a significant correlation was observed between let-7 and K-ras gene expression (r=-0.6336) in tumor tissues. Patients with a low let-7 expression had a significantly shorter survival than those with a moderate let-7 expression (P<0.05). Patients with a high K-ras expression also had a significantly shorter survival than those with a moderate K-ras expression (P<0.05). In conclusion, a low expression of let-7 and a high expression of K-ras are correlated with the pathogenesis and prognosis of NSCLC.
为检测非小细胞肺癌（NSCLC）中致死-7（let-7）和K-ras基因的表达水平并探讨其意义，采用逆转录-聚合酶链反应法检测31例NSCLC患者癌组织及癌旁正常组织中let-7和K-ras的表达。我们对31例NSCLC患者样本的检测结果显示，67.74%的患者let-7表达水平低（P<0.05），64.52%的患者K-ras表达水平高（P<0.05），且在肿瘤组织中let-7与K-ras基因表达之间存在显著相关性（r=-0.6336）。let-7表达水平低的患者生存时间显著短于let-7表达水平中等的患者（P<0.05）。K-ras表达水平高的患者生存时间也显著短于K-ras表达水平中等的患者（P<0.05）。总之，let-7低表达和K-ras高表达与NSCLC的发病机制及预后相关。
