Sadamori N, Yamaguchi K, Hakariya S, Mine M, Hayashibara T, Kawachi T, Sasagawa I, Iwasaki H, Kinoshita H, Hayashi K
Department of Internal Medicine, Nagasaki University School of Medicine.
Rinsho Ketsueki. 1990 Nov;31(11):1806-11.
To clarify the clinical significance of serum deoxythymidine kinase in various hematological disorders, basic examination was performed of a newly developed radioenzyme assay kit for TK (Prolifigen TK kit "Daiichi") using 125I-iodo-deoxyuridine as a substrate. The assessment of the standard curve, and the results of recovery test, dilution test, and precision and reproducibility were satisfactory. Among several anticoagulant agents tested, heparin was the best one showing no influence on the measurement of TK level. Based on these results, serum TK level was measured in 140 healthy adults as a control group. There was no difference by age and sex. The serum TK level of normal control was 3.1 +/- 1.2 U/l, with an upper limit of 5.5 U/l.
为阐明血清脱氧胸苷激酶在各种血液系统疾病中的临床意义，使用125I-碘脱氧尿苷作为底物，对新开发的用于胸苷激酶的放射酶分析试剂盒（Prolifigen TK试剂盒“第一制药”）进行了基础检测。标准曲线的评估以及回收率测试、稀释测试、精密度和重现性的结果均令人满意。在测试的几种抗凝剂中，肝素是最佳选择，对胸苷激酶水平的测量没有影响。基于这些结果，对140名健康成年人作为对照组测量了血清胸苷激酶水平。年龄和性别之间没有差异。正常对照组的血清胸苷激酶水平为3.1±1.2 U/l，上限为5.5 U/l。
