Department of Chemistry, University of California, Berkeley, CA 94720, United States.
Curr Opin Struct Biol. 2012 Dec;22(6):743-9. doi: 10.1016/j.sbi.2012.07.011. Epub 2012 Aug 4.
The ribosome undergoes numerous large-scale conformational changes during protein synthesis, but the molecular basis for these changes have been unclear. Recent cryo-electron microscopic and X-ray crystallographic structures of both the bacterial and eukaryotic ribosome now provide snapshots of the wide range of motions that occur within the ribosome. X-ray crystallographic structures of the ribosome have also pinpointed local deformations in ribosomal RNA that occur when the two ribosomal subunits rotate with respect to each other. These structural results establish the foundation for unraveling the mechanics of the ribosome that are universal, and those that differ in bacteria and eukaryotes.
核糖体在蛋白质合成过程中经历许多大规模构象变化，但这些变化的分子基础尚不清楚。最近的细菌和真核核糖体的冷冻电子显微镜和 X 射线晶体结构为核糖体内部发生的广泛运动提供了快照。核糖体的 X 射线晶体结构还确定了当两个核糖体亚基相对于彼此旋转时，核糖体 RNA 中发生的局部变形。这些结构结果为揭示核糖体的力学奠定了基础，这些力学在细菌和真核生物中是普遍存在的，而在细菌和真核生物中则有所不同。
