mRNA-tRNA 易位中间产物的结构特征。
Structural characterization of mRNA-tRNA translocation intermediates.
机构信息
Structural Biology Unit, CIC-bioGUNE, Bizkaia Technology Park, Derio 48160, Basque Country, Spain.
出版信息
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6094-9. doi: 10.1073/pnas.1201288109. Epub 2012 Mar 30.
Abstract
Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.
摘要
利用冷冻电镜分析野生型大肠杆菌的预转移样本，揭示了在转位的第一阶段存在先前未观察到的细菌核糖体中间状态，其特征为中间亚基旋转、L1 茎位置和 tRNA 构象的变化。此外，我们以定量的方式描述了结构域的重排，这使我们能够描述核糖体构象重排的连续性和协调性，以及 tRNA 与核糖体结合构象的相关变化。这些结果与核糖体作为一种分子机器的观点一致，它利用布朗运动通过一系列构象的逐步变化来达到功能上的有效状态。
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