Abramov Yoram, Hirsch Emmet, Ilievski Vladimir, Goldberg Roger P, Botros Sylvia M, Sand Peter K
Division of Urogynecology and Reconstructive Pelvic Surgery, Carmel Medical Center, Technion University, Rappaport Faculty of Medicine, Haifa, Israel.
Int Urogynecol J. 2013 Apr;24(4):671-5. doi: 10.1007/s00192-012-1905-x. Epub 2012 Aug 8.
Reconstructive pelvic surgery outcome is closely related to the vaginal and pelvic wound healing processes. Transforming growth factor beta 1 (TGF-β1) is a principal mediator of wound repair in dermal tissue. We sought to assess this factor's expression in vaginal and dermal surgical wound repair in the rabbit.
We excised bilateral 6-mm full-thickness circular segments from the abdominal skin and vagina in 36 New Zealand White (NZW) nulliparous female rabbits. Animals were sacrificed before, on the day of, and 4, 7, 10, 14, 21, 28, and 35 days after tissue wounding, and their wounds were assessed for surface area and TGF-β1 gene transcription by real-time polymerase chain reaction (PCR).
In both the abdominal skin and vagina, TGF-β1 gene transcription increased immediately after tissue injury, reaching maximal levels on days 4-7, and decreased shortly thereafter, attaining minimal values on day 35. A significant correlation between TGF-β1 expression and the wound's closure rate was found in both tissues.
TGF-β1 gene transcription significantly correlates with the surgical vaginal and dermal wound closure rate, implying that this factor is involved in the process of wound repair in both tissues.
盆腔重建手术的结果与阴道及盆腔伤口愈合过程密切相关。转化生长因子β1（TGF-β1）是真皮组织伤口修复的主要介质。我们旨在评估该因子在兔阴道和皮肤手术伤口修复中的表达情况。
我们从36只新西兰白兔未生育的雌性兔的腹部皮肤和阴道切除双侧6毫米全层圆形组织块。在组织损伤前、损伤当天以及损伤后4、7、10、14、21、28和35天处死动物，通过实时聚合酶链反应（PCR）评估其伤口的表面积和TGF-β1基因转录情况。
在腹部皮肤和阴道中，组织损伤后TGF-β1基因转录立即增加，在第4 - 7天达到最高水平，此后不久下降，在第35天达到最低值。在这两种组织中均发现TGF-β1表达与伤口闭合率之间存在显著相关性。
TGF-β1基因转录与手术阴道和皮肤伤口闭合率显著相关，这意味着该因子参与了这两种组织的伤口修复过程。
