The Medical School of Ningbo University, Ningbo, 315211, China.
J Mater Sci Mater Med. 2018 Sep 8;29(9):150. doi: 10.1007/s10856-018-6158-x.
In this work, two kinds of hyaluronic acid (HA)-based hydrogels were fabricated: one is made from physical freezing-thawing of HA solution (HA1), and the other is from chemical cross-linking of HA and polysaccharide (HA2). They were applied to repair full-thickness skin defects with New Zealand rabbits as the test animals, using powder HA and cotton dress as the references. The wound starts to heal after wounds were disinfected with iodine followed by coating with HA2, HA1, HA and cotton dress (the control), respectively. They were recorded as 4 treatments (groups), HA2, HA1, HA and the control. The healing progress was followed and tested in the duration of 56 days, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, HA2 was the most promising treatment in promoting the wound healing with least serious scar formation. Immunochemistry analyses and real-time PCR tests of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-β1), exhibited that HA2 enhanced VEGF and α-SMA secretion but reduced TGF-β1 expression at early stage, which alleviated the wound inflammation, improved the skin regeneration and relieved the scar formation.
在这项工作中，制备了两种基于透明质酸（HA）的水凝胶：一种是通过 HA 溶液的物理冷冻-解冻制备的（HA1），另一种是通过 HA 和多糖的化学交联制备的（HA2）。以新西兰兔为实验动物，采用粉末 HA 和棉敷料作为对照，将其应用于修复全层皮肤缺损。用碘消毒伤口后，分别用 HA2、HA1、HA 和棉敷料（对照）进行涂层，伤口开始愈合。它们被记录为 4 种治疗（组），HA2、HA1、HA 和对照。在 56 天的时间里，我们对愈合进展进行了跟踪和测试，并探索了其生物修复机制。从伤口面积的变化、白细胞（WBC）的测量和 H&E 染色来看，HA2 在促进伤口愈合方面最有前途，形成的疤痕最少。对参与伤口愈合的生物因子血管内皮生长因子（VEGF）、α-平滑肌肌动蛋白（α-SMA）和转化生长因子β-1（TGF-β1）的免疫化学分析和实时 PCR 检测表明，HA2 能增强 VEGF 和 α-SMA 的分泌，但在早期能降低 TGF-β1 的表达，从而减轻伤口炎症，促进皮肤再生，减轻疤痕形成。
