沙眼衣原体富含半胱氨酸的外膜蛋白在生物变种间表现出补偿性序列变化。

Cysteine-rich outer membrane proteins of Chlamydia trachomatis display compensatory sequence changes between biovariants.

作者信息

Allen J E, Cerrone M C, Beatty P R, Stephens R S

机构信息

Department of Biomedical and Environmental Health Sciences, University of California, Berkeley 94720.

出版信息

Mol Microbiol. 1990 Sep;4(9):1543-50. doi: 10.1111/j.1365-2958.1990.tb02065.x.

DOI:10.1111/j.1365-2958.1990.tb02065.x

PMID:2287277

Abstract

Two cysteine-rich proteins of Chlamydia trachomatis are essential structural components of the unique outer membrane of the infectious elementary body. These 58,000 (outer membrane protein 2; OMP2) and 15,000 (OMP3) proteins also differ structurally and chemically between biovariants that differ in invasive capability. We have identified the gene for OMP3 and sequenced both trachoma and lymphogranuloma venereum (LGV) omp3 genes. We have previously sequenced omp2 from the LGV biovar and now describe the omp2 sequence for a trachoma biovariant. Amino acid sequence differences between biovariants were few but, significantly, these changes have altered the charge of both OMP2 and OMP3 such that the net charge of each protein differs between biovariants. These compensatory charge alterations have implications for the outer membrane organization of these proteins. In addition, examination of the OMP3 sequence suggests that OMP3 may be a lipoprotein.

摘要

沙眼衣原体的两种富含半胱氨酸的蛋白质是感染性原体独特外膜的重要结构成分。这两种蛋白质分子量分别为58,000（外膜蛋白2；OMP2）和15,000（OMP3），在侵袭能力不同的生物变种之间，其结构和化学性质也存在差异。我们已经鉴定出OMP3的基因，并对沙眼和性病性淋巴肉芽肿（LGV）的omp3基因进行了测序。我们之前已经对LGV生物变种的omp2进行了测序，现在描述沙眼生物变种的omp2序列。生物变种之间的氨基酸序列差异很少，但重要的是，这些变化改变了OMP2和OMP3的电荷，使得每种蛋白质在生物变种之间的净电荷不同。这些补偿性的电荷改变对这些蛋白质的外膜组织有影响。此外，对OMP3序列的研究表明，OMP3可能是一种脂蛋白。