基于宽视场荧光显微镜并带有激光照明及散斑抑制装置的微阵列分析仪。
Microarray analyzer based on wide field fluorescent microscopy with laser illumination and a device for speckle suppression.
作者信息
Lysov Yuri, Barsky Victor, Urasov Dmitriy, Urasov Roman, Cherepanov Alecksey, Mamaev Dmitryi, Yegorov Yegor, Chudinov Alexander, Surzhikov Sergey, Rubina Alla, Smoldovskaya Olga, Zasedatelev Alexander
机构信息
Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilova str. 32, Moscow, Russia.
出版信息
Biomed Opt Express. 2017 Oct 3;8(11):4798-4810. doi: 10.1364/BOE.8.004798. eCollection 2017 Nov 1.
A microarray analyzer was developed to obtain images and measure the fluorescence intensity of microarrays at three wavelengths from 380 nm to 850 nm. The analyzer contains lasers to excite fluorescence, barrier filters, optics to project images on an image detector, and a device for suppressing laser speckles on the microarray support. The speckle suppression device contains a fibre-optic bundle and a rotating mirror positioned in a way to change the distance between the bundle butt and mirror surface during each mirror revolution. The analyzer provides for measurements with accuracy within ± 5%. Obtaining images at several exposure times allowed a significant expansion in the range of measured fluorescence intensities. The analyzer is useful for high throughput analysis of the same type of microarrays.
开发了一种微阵列分析仪，用于获取图像并在380纳米至850纳米的三个波长下测量微阵列的荧光强度。该分析仪包括用于激发荧光的激光器、阻挡滤光片、将图像投射到图像探测器上的光学器件，以及用于抑制微阵列支撑物上激光散斑的装置。散斑抑制装置包含一个光纤束和一个旋转镜，其定位方式是在每次镜旋转期间改变束端与镜表面之间的距离。该分析仪的测量精度在±5%以内。在几个曝光时间获取图像使得可测量的荧光强度范围显著扩大。该分析仪可用于对同一类型微阵列的高通量分析。
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