Jerant-Patić V, Ziramov J, Vujkov V, Hrnjaković-Cvjetković I, Popović S
Institut za zdravstvenu zastitu, N. Sad.
Med Pregl. 1990;43(5-6):236-41.
A random specimen of 288 women was examined by the use of the indirect immunofluorescent test (IIF) for the proof of the Chlamydia trachomatis antigen in endocervical swabs (by the help of monoclonal antibodies), and with the ELISA test for the proof of IgG antibodies against Chlamydia trachomatis in serums, and the complement--fixation reaction for the proof of antibodies against the Chlamydia-group antigen. These women were previously examined anamnestically and clinically in detail. The Chlamydia trachomatis infection was determined in 29.51% of cases, with a high percentage of positive results in all age groups. These infections were more frequently proved, to a considerable extent, in women with the diagnosis of sterility than in all other examined women, and in relation to all other inflammatory processes it was most frequently diagnosed in women with uterine cervix changes (endocervicitis and erythroplasia). A high percentage of Chlamydia positive subjects (40.91%) was established in those examined women which had pathological pregnancies and births in their past medical histories. The humoral immune response to the presence of Chlamydia trachomatis infections was proven in 70.59% of cases. Presented and analyzed are the diagnostic possibilities of the used diagnostic tests, where the combination of the IIF and ELISA test has shown to be the best.(ABSTRACT TRUNCATED AT 250 WORDS)
采用间接免疫荧光试验(IIF)(借助单克隆抗体)检测288名女性的随机样本宫颈拭子中的沙眼衣原体抗原,采用酶联免疫吸附测定(ELISA)检测血清中抗沙眼衣原体IgG抗体,并采用补体结合反应检测抗衣原体属抗原的抗体。这些女性之前已进行过详细的病史询问和临床检查。沙眼衣原体感染率为29.51%,各年龄组阳性率均较高。在很大程度上,不育症女性中这种感染的检出率高于所有其他受检女性,并且与所有其他炎症过程相比,子宫颈病变(宫颈炎和增殖)女性中最常诊断出这种感染。过去有病理妊娠和分娩史的受检女性中,沙眼衣原体阳性率较高(40.91%)。70.59%的病例证实了对沙眼衣原体感染存在体液免疫反应。文中展示并分析了所用诊断试验的诊断可能性,其中IIF和ELISA试验联合使用显示效果最佳。(摘要截选至250词)