Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College/Chinese Academy of Medical Sciences, Beijing 100730, China.
Virol J. 2012 Aug 8;9:153. doi: 10.1186/1743-422X-9-153.
Human papillomavirus (HPV) infection causes cervical cancer and premalignant lesions of the cervix. Prevalence of HPV infection and HPV genotypes vary among different regions. However there is no data on the prevalence of HPV infection and HPV genotypes from southwest China. This study was undertaken to determine the prevalence of and risk factors for HR-HPV infection in Qujing of Yunnan province, southwest China to provide comprehensive baseline data for future screening strategies.
A sample of 5936 women was chosen by the multi-stage stratified cluster sampling method with selection probabilities proportional to size (PPS). An epidemiological questionnaire was conducted via a face-to-face interview and cervical specimens were taken for HPV DNA testing by Digene Hybrid Capture 2 (HC2) test. HPV Genotyping Reverse Hybridization Test was used for HPV genotyping. Proportions were compared by Chi-squared tests, and logistic regression was utilized to evaluate risk factors.
The median age was 38 years and the inter-quartile range was from 31 years to 47 years. 97.3% of the study population was Han nationality. Overall prevalence of HR-HPV infection was 8.3% (494/5936) and bimodal age distribution of HPV infection was observed. The five most prevalent HR-HPV genotypes were HPV-16(3.4%), HPV-56(1.7%), HPV-58(1.4%), HPV-33(1.2%) and HPV-52(0.88%). Multiple HPV infections were identified in 50.5% (208/412) of the positive genotyping specimens. Multivariate logistic regression model indicated that parity (OR = 1.35, 95% CI: 1.18-1.53, p < 0.0001) was a risk factor for HR-HPV infection, and age of 50-65 years (OR = 0.60, 95% CI: 0.45-0.80, p = 0.0005), being married or in stable relationship (OR = 0.55, 95% CI: 0.31-0.96, p = 0.035) were protective factors.
This study provided baseline data on HR-HPV prevalence in the general female population in Qujing of Yunnan province, southwest China. The finding of multiple HPV infections and bimodal age distribution revealed that HPV screening is necessary for perimenopausal women in future.
人乳头瘤病毒（HPV）感染可导致宫颈癌和宫颈前病变。HPV 感染的流行率和 HPV 基因型在不同地区有所不同。然而，目前还没有来自中国西南部曲靖地区 HPV 感染和 HPV 基因型流行率的数据。本研究旨在确定云南省曲靖市 HR-HPV 感染的流行率和危险因素，为未来的筛查策略提供全面的基线数据。
采用多阶段分层聚类抽样法（PPS）选择了 5936 名女性样本。通过面对面访谈进行了一项流行病学问卷调查，并通过 Digene Hybrid Capture 2（HC2）试验对宫颈标本进行 HPV DNA 检测。采用 HPV 基因分型反向杂交试验进行 HPV 基因分型。采用卡方检验比较比例，采用 logistic 回归评估危险因素。
中位年龄为 38 岁，四分位间距为 31 岁至 47 岁。研究人群 97.3%为汉族。HR-HPV 感染总流行率为 8.3%（494/5936），HPV 感染呈双峰年龄分布。最常见的 5 种 HR-HPV 基因型为 HPV-16（3.4%）、HPV-56（1.7%）、HPV-58（1.4%）、HPV-33（1.2%）和 HPV-52（0.88%）。在 412 份阳性基因分型标本中，发现 50.5%（208/412）存在多重 HPV 感染。多因素 logistic 回归模型表明，产次（OR=1.35，95%CI：1.18-1.53，p<0.0001）是 HR-HPV 感染的危险因素，年龄在 50-65 岁（OR=0.60，95%CI：0.45-0.80，p=0.0005）、已婚或稳定关系（OR=0.55，95%CI：0.31-0.96，p=0.035）是保护因素。
本研究提供了云南省曲靖市普通女性人群 HR-HPV 流行率的基线数据。多重 HPV 感染和双峰年龄分布的发现表明，未来围绝经期妇女需要进行 HPV 筛查。
