Li Ying, Wang Yipeng, Jia Chanwei, Ma Yanmin, Lan Yonglian, Wang Shuyu
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
Cancer Genet Cytogenet. 2008 Apr 1;182(1):12-7. doi: 10.1016/j.cancergencyto.2007.12.007.
Human papillomavirus (HPV) is the main cause of cervical cancer. Blending multiplex polymerase chain reaction (PCR) amplification and multiplex hybridization to liquid bead microarray (LBMA), we detected and identified 25 common HPV genotypes using type-specific primers for HPV E6 and E7 genes in cervical lesions of northern Chinese patients. Of the 511 cervical samples, 349 (68.3%) were found to be HPV positive by HPV-LBMA. The distribution was 22 HPV positive of 100 in the control group (22%), 41 of 80 with chronic cervicitis (51%), 80 of 99 with cervical intraepithelial neoplasia (CIN) I (81%), 46 of 56 with CIN II (82%), 67 of 74 with CIN III (90%), and 93 of 102 with invasive cervical carcinoma (91%). HPV-16 was the most frequent genotype in the CIN and cervical cancer groups. The most common genotypes were HPV-16 (28%), HPV-58 (14%), HPV-52 (14%), HPV-18 (8%), and HPV-33 (7%) in the CIN group, and HPV-16 (63%), HPV-52 (9%), HPV-18 (7%), HPV-58 (7%), and HPV-33 (5%) in the cervical cancer group. HPV-LBMA found multiple genotypes in 1 of 22 control (4%), 64 of 193 CIN (33%), and 22 of 93 cervical cancer (24%). The HPV-LBMA results were compatible with those of PCR and DNA sequencing. HPV-LBMA is a simple, high-throughput method that provides useful information on viral genotype and multiple HPV infections in cervical lesions. In northern China, the most common high-risk HPV genotypes seem to be HPV types 16, 58, 52, 18, and 33. Genetic information on HPV in cervical specimens could provide particular benefits in the management of cervical lesions.
人乳头瘤病毒（HPV）是宫颈癌的主要病因。通过将多重聚合酶链反应（PCR）扩增与液体微珠芯片（LBMA）多重杂交相结合，我们使用针对HPV E6和E7基因的型特异性引物，在中国北方患者的宫颈病变中检测并鉴定了25种常见的HPV基因型。在511份宫颈样本中，HPV-LBMA检测发现349份（68.3%）呈HPV阳性。分布情况为：对照组100份中有22份HPV阳性（22%），慢性宫颈炎患者80份中有41份（51%），宫颈上皮内瘤变（CIN）I级患者99份中有80份（81%），CIN II级患者56份中有46份（82%），CIN III级患者74份中有67份（90%），浸润性宫颈癌患者102份中有93份（91%）。HPV-16是CIN组和宫颈癌组中最常见的基因型。CIN组中最常见的基因型为HPV-16（28%）、HPV-58（14%）、HPV-52（14%）、HPV-18（8%）和HPV-33（7%），宫颈癌组中为HPV-16（63%）、HPV-52（9%）、HPV-18（7%）、HPV-58（7%）和HPV-33（5%）。HPV-LBMA在22份对照组样本中的1份（4%）、193份CIN样本中的64份（33%）以及93份宫颈癌样本中的22份（24%）中检测到多种基因型。HPV-LBMA的结果与PCR和DNA测序结果相符。HPV-LBMA是一种简单的高通量方法，可提供宫颈病变中病毒基因型和多种HPV感染的有用信息。在中国北方，最常见的高危HPV基因型似乎是HPV 16、58、52、18和33型。宫颈标本中HPV的基因信息在宫颈病变的管理中可能具有特殊益处。
