细胞因子基因多态性与运动对老年女性临床和炎症参数影响的相互作用：一项随机对照试验的研究方案。
Interaction between cytokine gene polymorphisms and the effect of physical exercise on clinical and inflammatory parameters in older women: study protocol for a randomized controlled trial.
机构信息
Department of Physical Therapy, Graduate Program in Rehabilitation Sciences, School of Physical Education, Physical Therapy and Occupational Therapy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
出版信息
Trials. 2012 Aug 8;13:134. doi: 10.1186/1745-6215-13-134.
BACKGROUND
Aging is associated with chronic low-grade inflammatory activity with an elevation of cytokine levels. An association between regular physical activity and reduction of blood levels of anti-inflammatory cytokines is demonstrated in the literature pointing to an anti-inflammatory effect related to exercise. However, there is no consensus regarding which type of exercise and which parameters are the most appropriate to influence inflammatory markers. Evidence indicates that the single nucleotide polymorphism (SNP) can influence the synthesis of those cytokines affecting their production.
METHODS/DESIGN: The design of this study is a randomized controlled trial. The aim of this study is to investigate the interaction between the cytokine genes SNP and the effect of physical activity on older women. The main outcomes are: serum levels of sTNFR-1, sTNFR-2, interleukin (IL)-6, IL-10, measured by the ELISA method; genotyping of tumor necrosis factor- (TNF)-alpha (rs1800629), IL6 (rs1800795), IL10 (rs1800896) by the TaqMan Method (Applied Biosystems, Foster City, CA, USA); and physical performance assessed by Timed Up and Go and 10-Meter Walk Tests. Secondary outcomes include: Geriatric Depression Scale, Perceived Stress Scaleand aerobic capacity, assessed by the six-minute walk; and lower limb muscle strength, using an isokinetic dinamometer (Biodex Medical Systems, Inc., Shirley, NY,USA). Both exercise protocols will be performed three times a week for 10 weeks, 30 sessions in total.
DISCUSSION
Investigating the interaction between genetic factors and exercise effects of both protocols of exercise on the levels of inflammatory cytokine levels can contribute to guide clinical practice related to treatment and prevention of functional changes due to chronic inflammatory activity in older adults. This approach could develop new perspectives on preventive and treatment proposals in physical therapy and in the management of the older patient.
TRIAL REGISTRATION
(ReBEC) RBR9v9cwf.
背景
衰老与慢性低度炎症活动有关，其特征是细胞因子水平升高。文献中已经证明，有规律的体育活动与抗炎细胞因子血水平降低之间存在关联，这表明运动具有抗炎作用。然而，对于哪种类型的运动和哪些参数最适合影响炎症标志物，目前尚无共识。有证据表明，单核苷酸多态性 (SNP) 可以影响这些细胞因子的合成，从而影响其产生。
方法/设计：本研究的设计是一项随机对照试验。本研究的目的是调查细胞因子基因 SNP 与体力活动对老年女性的影响之间的相互作用。主要结局指标是：采用 ELISA 法测定血清可溶性肿瘤坏死因子受体-1 (sTNFR-1)、sTNFR-2、白细胞介素 (IL)-6、IL-10 的水平；采用 TaqMan 法（Applied Biosystems，福斯特城，加利福尼亚州，美国）对肿瘤坏死因子-α (TNF)-alpha(rs1800629)、IL6(rs1800795)、IL10(rs1800896) 进行基因分型；采用计时起立行走测试和 10 米步行测试评估身体机能。次要结局指标包括：使用六分钟步行试验评估老年抑郁量表、感知压力量表和有氧能力；使用等动测力计（Biodex Medical Systems，Inc.，雪利，纽约，美国）评估下肢肌肉力量。两种运动方案均每周进行 3 次，共 10 周，共 30 次。
讨论
研究遗传因素与两种运动方案的运动效果之间的相互作用，对炎症细胞因子水平的影响，可以为指导与慢性炎症活动相关的老年患者功能变化的治疗和预防的临床实践提供依据。这种方法可以为物理治疗和老年患者管理方面的预防和治疗建议提供新的视角。
试验注册
（ReBEC）RBR9v9cwf。
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