Department of Physics, University of Oxford, Oxford, United Kingdom.
PLoS One. 2012;7(8):e42562. doi: 10.1371/journal.pone.0042562. Epub 2012 Aug 2.
Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches.
电解剖映射技术将心脏内电活动与解剖位置的关系进行了关联，成为评估复杂心律失常的重要临床工具。心脏电生理学的光学映射技术将解剖结构和生理功能的高时空分辨率与快速、同步的数据采集相结合。如果将其应用于临床环境中，这将提高临床心律失常干预的诊断潜力和治疗效果。本研究旨在使用大鼠模型探索这种用途。为此，我们提出了一种单相机成像和多个发光二极管照明系统，降低了心脏光学映射的经济和技术实施障碍。结合适用于血灌注组织的红移钙染料和新型近红外电压敏感染料，我们证明了在体多参数哺乳动物心脏成像的可行性。我们的方法结合了电生理相关参数的记录和结构基质的观察，并且在原理上可以适应经导管经皮的方法。
