Zeng Jun, Mahou Pierre, Schanne-Klein Marie-Claire, Beaurepaire Emmanuel, Débarre Delphine
Biomed Opt Express. 2012 Aug 1;3(8):1898-913. doi: 10.1364/BOE.3.001898. Epub 2012 Jul 20.
We demonstrate a simple method for mapping optical aberrations with 3D resolution within thick samples. The method relies on the local measurement of the variation in image quality with externally applied aberrations. We discuss the accuracy of the method as a function of the signal strength and of the aberration amplitude and we derive the achievable resolution for the resulting measurements. We then report on measured 3D aberration maps in human skin biopsies and mouse brain slices. From these data, we analyse the consequences of tissue structure and refractive index distribution on aberrations and imaging depth in normal and cleared tissue samples. The aberration maps allow the estimation of the typical aplanetism region size over which aberrations can be uniformly corrected. This method and data pave the way towards efficient correction strategies for tissue imaging applications.
我们展示了一种在厚样本中以三维分辨率绘制光学像差的简单方法。该方法依赖于通过外部施加像差来局部测量图像质量的变化。我们讨论了该方法的精度与信号强度和像差幅度的函数关系，并推导了所得测量结果可实现的分辨率。然后，我们报告了在人体皮肤活检样本和小鼠脑切片中测得的三维像差图。根据这些数据，我们分析了组织结构和折射率分布对正常和透明组织样本中的像差及成像深度的影响。像差图有助于估计可对像差进行均匀校正的典型消球差区域大小。这种方法和数据为组织成像应用的高效校正策略铺平了道路。
