Cueto P H, Giulietti A M, dos Santos C, Méndez B S
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1990 Apr-Jun;22(2):57-61.
Butanol high producing mutants of a solventogenic Clostridium sp. capable of degrading olive black water, were selected according to ethanol or butanol resistance after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Mutants were quickly screened from isolated colonies and then characterized in standard culture conditions.
一株能够降解橄榄黑水的产溶剂梭菌属丁醇高产突变体，在用N-甲基-N'-硝基-N-亚硝基胍处理后，根据对乙醇或丁醇的抗性进行筛选。从分离出的菌落中快速筛选出突变体，然后在标准培养条件下对其进行表征。
