低水平的肝细胞 HIV 感染。

Low-level HIV infection of hepatocytes.

机构信息

Division of Digestive Diseases, University of Cincinnati College of Medicine, and Cincinnati Children’s Hospital Medical Center, ML 0595, 231 Albert Sabin Way, Cincinnati, OH 45267, USA.

出版信息

Virol J. 2012 Aug 9;9:157. doi: 10.1186/1743-422X-9-157.

BACKGROUND

There are only limited data on whether HIV infection occurs within the liver; therefore, we explored early and late stages of the HIV life cycle in two hepatocyte cell lines--Huh7.5 and Huh7.5JFH1--as well as in primary human hepatocytes.

RESULTS

Integrated HIV DNA was detected in Huh7.5 and Huh7.5JFH1 cells, as well as in primary hepatocytes, and was inhibited by the integrase inhibitor raltegravir in a dose-dependent manner. HIV p24 protein was also detected in cell culture supernatants at days 1, 3, 5, and 7 post-infection and was inhibited by AZT, although levels were modest compared to those in a lymphocyte cell line. Culture supernatants from HIV-infected hepatocytes were capable of infecting a non-hepatic HIV indicator cell line.

CONCLUSIONS

These results indicating low-level HIV replication in hepatoctyes in vitro complement evidence suggesting that HIV has deleterious effects on the liver in vivo.

背景

关于 HIV 感染是否发生在肝脏内，目前仅有有限的数据。因此，我们在两种肝细胞系——Huh7.5 和 Huh7.5JFH1 以及原代人肝细胞中，探究了 HIV 生命周期的早期和晚期阶段。

结果

整合的 HIV DNA 在 Huh7.5 和 Huh7.5JFH1 细胞以及原代肝细胞中被检测到，并被整合酶抑制剂拉替拉韦以剂量依赖的方式抑制。HIV p24 蛋白也在感染后第 1、3、5 和 7 天的细胞培养上清液中被检测到，并被 AZT 抑制，尽管与淋巴细胞细胞系相比，水平较低。来自感染 HIV 的肝细胞的培养上清液能够感染非肝细胞 HIV 指示细胞系。