Mills B G, Singer F R, Weiner L P, Holst P A
Calcif Tissue Int. 1979 Nov 26;29(2):79-87. doi: 10.1007/BF02408061.
Cells obtained from surgical bone specimens of eight patients with Paget's disease of bone were maintained in culture for up to 8 months and seven passages. The doubling time during the period of maximal cell growth ranged from 4 to 12 days. Evidence consistent with the hypothesis that many of the cells were bone cells included the following: (a) histochemical techniques demonstrated staining of some cells for alkaline phosphatase or acid phosphatase and succinic dehydrogenase; (b) parathyroid extract stimulated increased uptake of 3H-thymidine and 3H-uridine; (c) parathyroid extract suppressed and salmon calcitonin stimulated uptake of 3H-proline; and (d) crystalline calcium deposits were found within cells and extracellularly. Ultrastructural analysis revealed that three of the eight cultures contained cells whose nuclei had inclusions which were almost identical to those found in the osteoclast nuclei of all patients with Paget's disease. The maintenance of cells derived from pagetic bone in long-term culture should aid in testing the hypothesis that Paget's disease represents a slow virus infection of bone.
从8例骨Paget病患者的手术骨标本中获取的细胞，在培养中维持了长达8个月并传代7次。细胞最大生长期间的倍增时间为4至12天。与许多细胞为骨细胞这一假说相符的证据如下：(a) 组织化学技术显示部分细胞对碱性磷酸酶、酸性磷酸酶和琥珀酸脱氢酶呈阳性染色；(b) 甲状旁腺提取物刺激3H-胸腺嘧啶核苷和3H-尿苷摄取增加；(c) 甲状旁腺提取物抑制而鲑鱼降钙素刺激3H-脯氨酸摄取；(d) 在细胞内和细胞外发现有结晶状钙沉积。超微结构分析显示，8个培养物中有3个含有细胞核内有包涵体的细胞，这些包涵体与所有骨Paget病患者破骨细胞核内的包涵体几乎相同。长期培养来自Paget骨的细胞应有助于检验骨Paget病代表骨的慢病毒感染这一假说。
