Forster M J, Lal H
Department of Pharmacology, Texas College of Osteopathic Medicine, Fort Worth 76107-2690.
Int J Dev Neurosci. 1990;8(6):679-87. doi: 10.1016/0736-5748(90)90062-7.
Separate age groups of autoimmune NZB/BINJ and non-autoimmune C57BL/BNNia mice were compared for habituation of locomotor activity and its retention over four separate testing sessions spaced at 24-hr intervals. A decline in locomotion (distance in cm) or in the time spent in the center zone as a function of sessions was taken to indicate retention for habituation to stimuli within the test apparatus. The time spent in the center zone decreased as a function of sessions in young and mature C57BL/6NNia mice but failed to show reliable between-session decreases in old (24-26-months) C57BL/6NNia mice. When compared with the old C57BL/6NNia mice, young NZB/BINJ mice showed similar impairments. Habituation of locomotion was present in all age groups of C57BL/6NNia mice, but absent in NZB/BINJ mice regardless of age. The retention impairments of 2-4 month old NZB/BINJ mice were attenuated when i.p. injections of 0.04-0.16 mg physostigmine/kg were given just following each habituation session. The effectiveness of physostigmine was substantially reduced when injections were delayed by 20 min or longer following each habituation session. The time-dependent reversal of the aged-like retention deficits by the cholinesterase inhibitor, physostigmine, suggests that cholinergic modulation of memory storage processes may be impaired in NZB/BINJ mice.
对自身免疫性NZB/BINJ小鼠和非自身免疫性C57BL/BNNia小鼠的不同年龄组进行比较，观察其运动活动的习惯化情况以及在间隔24小时的四个独立测试阶段中的保持情况。运动能力下降（以厘米为单位的距离）或在中央区域停留时间随测试阶段的变化被视为对测试装置内刺激的习惯化保持的指标。在年轻和成熟的C57BL/6NNia小鼠中，在中央区域停留的时间随测试阶段而减少，但在老年（24 - 26个月）C57BL/6NNia小鼠中，各测试阶段之间未显示出可靠的下降。与老年C57BL/6NNia小鼠相比，年轻的NZB/BINJ小鼠表现出类似的损伤。C57BL/6NNia小鼠的所有年龄组均存在运动习惯化，但NZB/BINJ小鼠无论年龄大小均不存在运动习惯化。当在每个习惯化阶段后立即腹腔注射0.04 - 0.16 mg/kg毒扁豆碱时，2 - 4月龄NZB/BINJ小鼠的保持损伤得到减轻。当在每个习惯化阶段后延迟20分钟或更长时间注射时，毒扁豆碱的有效性显著降低。胆碱酯酶抑制剂毒扁豆碱对类似老年的保持缺陷的时间依赖性逆转表明，NZB/BINJ小鼠中记忆存储过程的胆碱能调制可能受损。
