Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán 04510, Mexico, DF, Mexico.
Brain Res Bull. 2012 Nov 1;89(3-4):108-14. doi: 10.1016/j.brainresbull.2012.07.011. Epub 2012 Aug 3.
Pregnancy involves changes in brain function that implicate a re-organization in neuronal cytoskeleton. We analyzed the content of the microtubule associated protein Tau (65kDa isoform) and its phosphorylated form (PhosphoTau) in several rat brain regions throughout pregnancy and on day 2 of lactation by Western blot. In hypothalamus the content of Tau increased on days 2 and 18 of gestation compared with days 14, 21 and in lactation. PhosphoTau content increased throughout pregnancy. In preoptic area Tau content did not show significant changes throughout pregnancy or lactation, however, the content of PhosphoTau presented a decrease on day 21 of gestation. In hippocampus Tau content decreased on day 14 until day 21 compared with day 2 of gestation, however, in lactation day 2 the content of Tau increased meanwhile PhosphoTau content progressively increased throughout pregnancy. In frontal cortex Tau content decreased on day 21 of gestation compared with days 2, 14 and 18, with an increase in lactation, whereas PhosphoTau did not show significant changes. In cerebellum Tau protein decreased on days 14, 18 and 21 of pregnancy with an increase in lactation. PhosphoTau content increased throughout pregnancy and on day 2 of lactation. PhosphoTau/Tau ratio changes in each brain area along pregnancy and in lactation. Our data suggest that Tau expression and its phosphorylation pattern change in a tissue-dependent manner throughout pregnancy and the beginning of lactation in the rat brain.
妊娠涉及脑功能的变化，这暗示了神经元细胞骨架的重新组织。我们通过 Western blot 分析了妊娠和哺乳期大鼠大脑不同区域微管相关蛋白 Tau（65kDa 同工型）及其磷酸化形式（PhosphoTau）的含量。在妊娠第 2 天和第 18 天，与妊娠第 14 天、第 21 天和哺乳期相比，下丘脑 Tau 的含量增加。在妊娠期间，PhosphoTau 的含量增加。在视前区，Tau 的含量在整个妊娠和哺乳期没有明显变化，但在妊娠第 21 天，PhosphoTau 的含量下降。在海马中，Tau 的含量在妊娠第 14 天至第 21 天与妊娠第 2 天相比有所下降，但在哺乳期第 2 天，Tau 的含量增加，而 PhosphoTau 的含量则在整个妊娠期间逐渐增加。在额叶皮层中，与妊娠第 2 天、第 14 天和第 18 天相比，Tau 的含量在妊娠第 21 天下降，在哺乳期增加，而 PhosphoTau 没有明显变化。在小脑，Tau 蛋白在妊娠第 14、18 和 21 天下降，哺乳期增加。在整个妊娠期间和哺乳期第 2 天，PhosphoTau 的含量增加。在妊娠和哺乳期期间，每个脑区的 PhosphoTau/Tau 比值发生变化。我们的数据表明，Tau 的表达及其磷酸化模式在组织依赖性的方式下在整个妊娠和哺乳期大鼠大脑中发生变化。
