Programa de Pós-Graduação em Biologia Geral e Aplicada, Universidade Estadual Paulista-UNESP, Instituto de Biociências, Botucatu, São Paulo, Brazil.
J Ethnopharmacol. 2012 Sep 28;143(2):740-5. doi: 10.1016/j.jep.2012.07.044. Epub 2012 Aug 5.
Brassica oleracea L. var. acephala D.C. has been extensively used in Brazilian traditional medicine to treat gastric ulcer.
This study was conducted to evaluate the in vivo genotoxic and/or antigenotoxic potential of a Brassica oleraceae hydroalcoholic extract obtained from the leaves, in different cells of mice.
Analyses were performed using the comet assay, on leukocytes (collected 4 and 24 h after treatment), liver, brain, bone marrow and testicular cells (collected 24 h after treatment), and using the micronucleus test (MN) in bone marrow cells. Eight groups of albino Swiss mice were treated (N=6): control (C), positive control (doxorubicin 80 mg/kg (DXR)), and six experimental groups, which received 500, 1000 and 2000 mg/kg of Brassica oleraceae extract alone by gavage, while a further three groups received the same doses plus DXR (80 mg/kg). We calculated the damage scores, and their averages were compared by ANOVA followed by the Tukey test for multiple comparisons.
The results demonstrated that none of the tested doses of Brassica oleraceae extract showed genotoxic effects by the comet assay, or clastogenic effects by the MN test. On the other hand, for all cells evaluated, the three tested doses of the Brassica extract promoted inhibition of DNA damage induced by DXR.
Under our experimental conditions, Brassica oleraceae leaf extract showed no genotoxic or clastogenic effects in different cells of mice. However, it did show a significant decrease in DNA damage induced by doxorubicin. It is suggested that the antigenotoxic properties of this extract may be of great pharmacological importance, and may be beneficial for cancer prevention.
无头甘蓝（ Brassica oleracea L. var. acephala D.C.）在巴西传统医学中被广泛用于治疗胃溃疡。
本研究旨在评估从叶片中获得的甘蓝水醇提取物对不同细胞的体内遗传毒性和/或抗原毒性。
使用彗星试验在白细胞（处理后 4 小时和 24 小时采集）、肝脏、大脑、骨髓和睾丸细胞（处理后 24 小时采集）中分析，以及在骨髓细胞中进行微核试验（MN）。将 8 组白化瑞士小鼠（N=6）进行处理：对照组（C）、阳性对照组（多柔比星 80mg/kg（DXR））和 6 个实验组，分别通过灌胃给予 500、1000 和 2000mg/kg 的甘蓝提取物，另外 3 个组则给予相同剂量的提取物和 DXR（80mg/kg）。我们计算了损伤评分，并通过方差分析（ANOVA）和 Tukey 多重比较检验对平均值进行比较。
结果表明，通过彗星试验，甘蓝提取物的测试剂量均未显示遗传毒性，MN 试验也未显示出断裂剂效应。另一方面，对于所有评估的细胞，甘蓝提取物的三种测试剂量均可抑制 DXR 诱导的 DNA 损伤。
在我们的实验条件下，甘蓝叶片提取物在小鼠的不同细胞中未显示出遗传毒性或断裂剂效应。然而，它确实显示出对多柔比星诱导的 DNA 损伤有显著的抑制作用。这表明该提取物的抗原毒性特性可能具有重要的药理学意义，并可能有益于癌症预防。
