生姜水提取物对雄性白化小鼠地塞米松遗传毒性的抑制作用。
The Inhibitory Effect of Aqueous Ginger Extract on the Genotoxicity of Dexamethasone in Male Albino Mice.
作者信息
Al-Aamiri Aseel Raheem
机构信息
Department of Biology, College of Education, Al-Qadisiyah University, Al-Qadisiyah, Iraq.
出版信息
Rep Biochem Mol Biol. 2025 Jan;13(4):466-473. doi: 10.61186/rbmb.13.4.466.
BACKGROUND
Many natural substances generated from plant crude extracts have recently been shown to protect against the harmful effects of a variety of pollutants. Ginger () is a widely used spice and medicinal herb.
METHODS
To study the effect of aqueous ginger extract in inhibiting the genotoxicity of dexamethasone, we gave the first group dexamethasone (0.4 mg/kg) only. The second group was treated with an aqueous extract of ginger (50 mg/kg) only. The third group was treated with an aqueous extract of ginger followed by dexamethasone with a two-hour interval between doses. The last group was treated with dexamethasone and an aqueous ginger extract simultaneously. To perform genetic tests, we used mitotic index, chromosomal aberrations, and micronuclei tests.
RESULTS
After the treatment with dexamethasone, chromosome aberration and micronuclei formation were induced; however, after treatment with an aqueous extract of ginger, chromosomal aberrations and micronuclei were significantly reduced in male mice. The aqueous extract of ginger did not exhibit cytotoxicity and showed high inhibitory efficiency against the toxicity and mutagenicity of dexamethasone.
CONCLUSIONS
The aqueous extract of ginger plays a promising role in protecting somatic cells from the cytogenetic effects of dexamethasone, and it reduces chromosomal aberrations and micronuclei in male albino mice.
背景
最近研究表明，许多从植物粗提物中提取的天然物质能够抵御多种污染物的有害影响。生姜是一种广泛使用的香料和药草。
方法
为了研究生姜水提取物对抑制地塞米松遗传毒性的作用，我们对第一组仅给予地塞米松（0.4毫克/千克）。第二组仅用生姜水提取物（50毫克/千克）处理。第三组先用生姜水提取物处理，两小时后给予地塞米松。最后一组同时给予地塞米松和生姜水提取物。为了进行基因测试，我们采用了有丝分裂指数、染色体畸变和微核测试。
结果
地塞米松处理后，诱导了染色体畸变和微核形成；然而，用生姜水提取物处理后，雄性小鼠的染色体畸变和微核显著减少。生姜水提取物未表现出细胞毒性，并且对 地塞米松的毒性和致突变性显示出高抑制效率。
结论
生姜水提取物在保护体细胞免受地塞米松的细胞遗传学效应方面发挥着有前景的作用，并且它减少了雄性白化小鼠的染色体畸变和微核。
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