Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, Heilongjiang, China.
Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, Heilongjiang, China; Harbin Med Univ, Coll Pharm, Key Lab Gut Microbiota & Pharmacogen Heilongjiang, Harbin 150081, Heilongjiang, China.
Ultrason Sonochem. 2024 Dec;111:107083. doi: 10.1016/j.ultsonch.2024.107083. Epub 2024 Sep 25.
Phyllanthi Fructus (PF) is a valuable botanical resource with a long history of traditional use, known for potent antioxidant and anti-inflammatory effects attributed to its rich contents of bioactive compounds, particularly polyphenols. However, current extraction techniques limit the utilization of polyphenols from PF. This study aimed to achieve the maximum polyphenol yield and improve the antioxidant activity of PF extracts to promise PF's prospects for modern healthcare. Firstly, ultrasonic-assisted extraction (UAE) was employed to extract the polyphenols in PF and a combination of Plackett-Burman designs (PBD) and response surface methodology (RSM) was applied to optimize UAE's conditions. Next, cellular superoxide dismutase (SOD) and malondialdehyde (MDA) were used to assess the antioxidant activity of extracted polyphenols. Ultra-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF MS) was utilized to characterize polyphenol components in the PF extracts. Finally, network pharmacology and molecular docking analysis were performed to screen the potential target proteins of polyphenols from PF. As a result, the optimized polyphenol yield was 213.49 mg/g, and the antioxidant activities, measured by ability of DPPH scavenging, ABTS+ scavenging, and FRAP were 76.95 %, 2.24 mmol/g, 2.34 mmol/g, respectively. PF extracts also showed good antioxidant capabilities at cellular level. 26 polyphenol components were identified in the PF extracts. Among these, ellagic acid, myricetin, and eriodictyol may exert antioxidant effects by interacting with AKT serine/threonine kinase 1 (AKT1). In conclusion, our study provides valuable insights into the optimizing PF extraction and underscores its potential applications in enhancing natural polyphenols extraction using UAE with a combination of PBD and RSM. These findings offer a promising avenue for the development and utilization of PF, and could serve as a reference for similar extraction processes in the future.
藤黄果（PF）是一种具有悠久传统用途的有价值的植物资源，以其丰富的生物活性化合物含量而闻名，特别是多酚，具有强大的抗氧化和抗炎作用。然而，当前的提取技术限制了 PF 中多酚的利用。本研究旨在实现最大的多酚产量，并提高 PF 提取物的抗氧化活性，以保证 PF 在现代医疗保健中的前景。首先，采用超声辅助提取（UAE）提取 PF 中的多酚，并应用 Plackett-Burman 设计（PBD）和响应面法（RSM）对 UAE 的条件进行优化。接下来，细胞超氧化物歧化酶（SOD）和丙二醛（MDA）用于评估提取多酚的抗氧化活性。采用超高效液相色谱与四极杆飞行时间质谱联用（UPLC-Q-TOF MS）对 PF 提取物中的多酚成分进行表征。最后，进行网络药理学和分子对接分析，筛选 PF 多酚的潜在靶蛋白。结果，优化后的多酚得率为 213.49 mg/g，抗氧化活性，以 DPPH 清除能力、ABTS+清除能力和 FRAP 测定，分别为 76.95%、2.24 mmol/g 和 2.34 mmol/g。PF 提取物在细胞水平也表现出良好的抗氧化能力。在 PF 提取物中鉴定出 26 种多酚成分。其中，鞣花酸、杨梅素和圣草酚可能通过与 AKT 丝氨酸/苏氨酸激酶 1（AKT1）相互作用发挥抗氧化作用。总之，本研究为优化 PF 提取提供了有价值的见解，并强调了使用 PBD 和 RSM 组合的 UAE 增强天然多酚提取的潜在应用。这些发现为 PF 的开发和利用提供了有希望的途径，并可为未来类似的提取过程提供参考。
