Department of Pharmacognosy, National Research Centre, 33 El Buhouth St., Cairo, 12622, Egypt.
Department of Pharmacognosy and Natural Products, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr St., Shibîn el Kôm, 32511, Menoufia, Egypt.
Sci Rep. 2024 Oct 1;14(1):22765. doi: 10.1038/s41598-024-72153-0.
Several Pelargonium species are cultivated mainly to produce essential oils used in perfume industry and for ornamental purposes. Although the chemical composition and biological activities of their essential oils were extensively investigated, there is limited information about the chemical composition of their non-volatile constituents. In this study, we report an Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)-based metabolomics approach for the annotation and analysis of various metabolites in three species; P. graveolens, P. denticulatum, and P. fragrans utilizing The Global Natural Product Social Molecular Networking (GNPS) and multivariate data analyses for clustering of the metabolites. A total of 154 metabolites belonging to different classes were annotated. The three species are good sources of coumarins, benzoic acid derivatives, organic acids, fatty acids, and phospholipids. However, the highest level of flavonols (mono- and di-O-glycosides) and cinnamic acid derivatives was found in P. graveolens and P. denticulatum, whereas tannins and flavone C-glycosides were abundant in P. fragrans. The metabolic profiles clarified here provide comprehensive information on the non-volatile constituents of the three Pelargonium species and can be employed for their authentication and possible therapeutic applications.
几种天竺葵属植物主要被栽培用于生产香水工业用的精油和用于观赏目的。尽管它们的精油的化学成分和生物活性已经被广泛研究，但对于它们的非挥发性成分的化学成分信息却很有限。在这项研究中，我们报告了一种基于超高效液相色谱-质谱（UPLC-MS）的代谢组学方法，用于注释和分析三种植物（天竺葵、天竺葵和天竺葵）的各种代谢物；利用全球天然产物社交分子网络（GNPS）和多元数据分析对代谢物进行聚类。总共注释了 154 种属于不同类别的代谢物。这三个物种是香豆素、苯甲酸衍生物、有机酸、脂肪酸和磷脂的良好来源。然而，天竺葵和天竺葵中发现了最高水平的类黄酮（单和二-O-糖苷）和肉桂酸衍生物，而鞣质和黄酮 C-糖苷在天竺葵中含量丰富。这里阐明的代谢谱提供了关于三种天竺葵属植物非挥发性成分的综合信息，并可用于它们的鉴定和可能的治疗应用。
