Faculty of Medicine, Department of Obstetrics and Gynecology, Karadeniz Technical University, Trabzon, Turkey. drmaosmanaga @ gmail.com
Gynecol Obstet Invest. 2012;74(2):125-30. doi: 10.1159/000339134. Epub 2012 Aug 9.
BACKGROUND/AIMS: To evaluate the ovarian-protective effects of clotrimazole on ovarian ischemia/reperfusion injury in a rat ovarian-torsion model.
32 Sprague-Dawley rats were randomly divided into four groups: (1) ischemia group (n = 8) in which only left adnexal torsion was performed for 2 h, but no treatment was given; (2) vehicle group (n = 8) in which left adnexal torsion was performed for 2 h and at the end of 2 h ischemia polyethylene glycol (3% PEG, 1 ml, i.p.) was administered and a 24-hour reperfusion was continued; (3) clotrimazole group (n = 8) in which left adnexal torsion was performed for 2 h and at the end of 2 h ischemia clotrimazole (30 mg/kg, i.p.) was administered and a 24-hour reperfusion was continued, and (4) control group (sham-operated, n = 6) in which no adnexal torsion and no treatment were given. The criteria for ovarian ischemia were follicular cell degeneration, vascular congestion, hemorrhage and infiltration by inflammatory cells. Each specimen was scored for each criterion (0, none; 1, mild; 2, moderate; 3, severe).
Clotrimazole significantly decreased plasma levels of serum malondialdehyde, ischemia-modified albumin, and total oxidant status.
This study showed the ovarian-protective effects of clotrimazole on ovarian ischemia/reperfusion injury.
背景/目的：评估克霉唑在大鼠卵巢扭转模型中对卵巢缺血/再灌注损伤的卵巢保护作用。
32 只 Sprague-Dawley 大鼠随机分为四组：（1）缺血组（n = 8），仅行左侧附件扭转 2 h，但不给予任何治疗；（2）载体组（n = 8），行左侧附件扭转 2 h，在 2 h 缺血结束时给予聚乙二醇（3% PEG，1 ml，腹腔内注射），并继续 24 h 再灌注；（3）克霉唑组（n = 8），行左侧附件扭转 2 h，在 2 h 缺血结束时给予克霉唑（30 mg/kg，腹腔内注射），并继续 24 h 再灌注；（4）对照组（假手术，n = 6），不进行附件扭转和治疗。卵巢缺血的标准为卵泡细胞变性、血管充血、出血和炎症细胞浸润。每个标本根据每个标准（0，无；1，轻度；2，中度；3，重度）进行评分。
克霉唑显著降低了血清丙二醛、缺血修饰白蛋白和总氧化剂状态的血浆水平。
本研究显示克霉唑对卵巢缺血/再灌注损伤具有卵巢保护作用。
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