卵泡液蛋白含量（FSH、LH、PG4、E2 和 AMH）与极体非整倍体。
Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy.
机构信息
Service d'Histologie, Embryologie, Biologie de la Reproduction, Cytogénétique et Génétique Médicale, Centre Hospitalier Poissy St Germain en Laye, ART Center, Poissy and EA2493, Paris Ile de France, Ouest Faculty, Versailles University, 10, rue du Champ Gaillard, 78303 Poissy, France.
出版信息
J Assist Reprod Genet. 2012 Oct;29(10):1123-34. doi: 10.1007/s10815-012-9841-8. Epub 2012 Aug 14.
PURPOSE
Our objective was to identify a marker for oocyte aneuploidy in follicular fluid (FF) in women with an increased risk of oocyte aneuploidy after controlled ovarian hyperstimulation.
MATERIALS AND METHODS
Three groups of oocytes were constituted for polar body screening by FISH (chromosomes 13, 16, 18, 21 and 22): Group 1, advanced maternal age (n = 156); Group 2, implantation failure (i.e. no pregnancy after the transfer of more than 10 embryos; n = 101) and Group 3, implantation failure and advanced maternal age (n = 56). FSH and other proteins were assayed in the corresponding FF samples.
RESULTS
Of the 313 oocytes assessed, 35.78 % were abnormal. We found a significant difference between the follicular FSH levels in normal oocytes and abnormal oocytes (4.85 ± 1.75 IU/L vs. 5.41 ± 2.47 IU/L, respectively; p = 0.021). We found that the greater the number of chromosomal abnormalities per oocyte (between 0 and 3), the higher the follicular FSH level.
CONCLUSION
High FF FSH levels were associated with oocyte aneuploidy in women having undergone controlled ovarian hyperstimulation.
目的
我们的目的是在接受控制性卵巢超刺激的卵母细胞非整倍体风险增加的女性的卵泡液（FF）中识别卵母细胞非整倍体的标志物。
材料和方法
通过 FISH（染色体 13、16、18、21 和 22）对三组卵母细胞进行极体筛查：第 1 组，高龄产妇（n = 156）；第 2 组，着床失败（即移植超过 10 个胚胎后仍未怀孕；n = 101）和第 3 组，着床失败和高龄产妇（n = 56）。相应的 FF 样本中检测了 FSH 和其他蛋白质。
结果
在评估的 313 个卵母细胞中，有 35.78%是异常的。我们发现正常卵母细胞和异常卵母细胞的卵泡 FSH 水平之间存在显著差异（分别为 4.85 ± 1.75 IU/L 和 5.41 ± 2.47 IU/L，p = 0.021）。我们发现，每个卵母细胞的染色体异常数量越多（0 到 3 之间），卵泡 FSH 水平越高。
结论
在接受控制性卵巢超刺激的女性中，高 FF FSH 水平与卵母细胞非整倍体有关。
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