Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Biotechnol J. 2012 Sep;7(9):1147-55. doi: 10.1002/biot.201200169. Epub 2012 Aug 14.
Aspergilli are widely used as cell factories for the production of food ingredients, enzymes and antibiotics. Traditionally, improvement of these cell factories has been done using classical methods, that is, random mutagenesis and screening; however, advances in methods for performing directed genetic modifications has enabled the use of metabolic engineering strategies. Genome sequencing of Aspergilli was originally trailing behind developments in the field of bacteria and yeasts, but with the recent availability of genome sequences for several industrially relevant Aspergilli, it has become possible to implement systems biology tools to advance metabolic engineering. These tools include genome-wide transcription analysis and genome-scale metabolic models. Herein, we review achievements in the field and highlight the impact of Aspergillus systems biology on industrial biotechnology.
曲霉菌被广泛用作生产食品成分、酶和抗生素的细胞工厂。传统上，这些细胞工厂的改良是使用经典方法，即随机诱变和筛选；然而，定向遗传修饰方法的进步使得代谢工程策略得以应用。曲霉菌的基因组测序最初落后于细菌和酵母领域的发展，但随着最近几株工业相关曲霉菌的基因组序列的获得，已经有可能实施系统生物学工具来推进代谢工程。这些工具包括全基因组转录分析和基因组规模的代谢模型。本文综述了该领域的成就，并强调了曲霉系统生物学对工业生物技术的影响。
