Department of Microbiology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq.
Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Open Vet J. 2024 Sep;14(9):2334-2347. doi: 10.5455/OVJ.2024.v14.i9.22. Epub 2024 Sep 30.
Suppression of quorum sensing (QS) that regulates many virulence factors, including antimicrobial resistance, in bacteria may subject the pathogenic microbes to the harmful consequences of the antibiotics, increasing their susceptibility to such drugs.
The current study aimed to make an aqueous crude extract from the soil isolate with the use of the gas chromatography-mass spectrometry (GC-MS) technique for its analysis, and then, study the impact of the extract on clinical isolates of
Preparation of crude extracts from (both organic and aqueous), which were then analyzed by GC-MS to detect the bioactive ingredients. Furthermore, the extract's capability to interfere with both the expression of the QS of and its antibacterial resistance was examined.
The highest GC-MS peak (37.11%.) appeared for 1,3-benzodioxole, 4-methoxy-6-(2-propenyl), along with the presence of other components of antibacterial activities. When the aqueous extract was added to the culture of two multi-drug resistant (MDR) a significant reduction in the expression of the QS regulatory gene occurred indicating its interference with QS Moreover, upon adding the extract to the culture of (MDR) and then subjecting it to Amikacin and Colistin, already not effective on the bacteria, the isolates became more susceptible to these antibiotics showing zones of inhibition of 25 and 17 mm, respectively.
The crude aqueous extract of the soil isolate might be a potential producer of QS inhibitors with antibacterial activities that render the MDR more susceptible to antibiotics to whom they already exerted resistance.
抑制群体感应（QS）可以调节细菌中的许多毒力因子，包括抗生素耐药性，这可能使病原微生物承受抗生素的有害后果，增加它们对这些药物的敏感性。
本研究旨在利用气相色谱-质谱（GC-MS）技术从土壤分离株中制备水相粗提物并对其进行分析，然后研究该提取物对临床分离株的影响。
制备（有机和水相）粗提取物，然后用 GC-MS 分析以检测生物活性成分。此外，还研究了提取物干扰表达的能力QS 和它的抗菌耐药性。
GC-MS 峰最高（37.11%）出现在 1,3-苯并二恶唑，4-甲氧基-6-（2-丙烯基），同时存在其他具有抗菌活性的成分。当将水提物加入到两种多药耐药（MDR）的培养物中时，QS 调节基因的表达显著减少，表明其干扰了 QS。此外，当将提取物加入到 MDR 培养物中，然后用阿米卡星和粘菌素处理时，这些抗生素对细菌已经无效，分离株对这些抗生素的敏感性增加，分别显示出 25 和 17 毫米的抑制区。
土壤分离株的水相粗提物可能是一种潜在的 QS 抑制剂产生者，具有抗菌活性，使 MDR 对已经产生耐药性的抗生素更敏感。
