Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University (USMBA), Fez B.P. 1796, Morocco.
Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
Molecules. 2021 Nov 10;26(22):6780. doi: 10.3390/molecules26226780.
The study of bioactive molecules of natural origin is a focus of current research. and are two medicinal plants widely used by the Moroccan population in the traditional treatment of several pathologies linked to inflammation. This study aimed to evaluate the single and combined antioxidant, anti-inflammatory and analgesic effects of the essential oils extracted from these two medicinal plants, and also their potential toxicity. Essential oils were extracted using hydro-distillation in a Clevenger-type apparatus. The antioxidant activity was evaluated by two methods: the scavenging of the free radical DPPH, and the reduction in iron. Anti-inflammatory activity was evaluated by evaluating the edema development induced by carrageenan injecting, while the analgesic power was evaluated according to the number of abdominal contortions induced by the intraperitoneal injection of acetic acid (0.7%). The acute oral toxicity was performed to assess the potential toxicity of the studied EOs, followed by an analysis of the blood biochemical parameters. The results of the two antioxidant tests indicated that our extract mixture exhibits good iron reduction capacity and very interesting DPPH free radical scavenging power, with an IC of around 4.38 ± 0.98 μg/mL higher than that of the benchmark antioxidant, BHT. The anti-inflammatory test demonstrated that the mixture administered orally at a dose of 150 mg/kg has a better activity, exceeding that of 1% Diclofenac, with a percentage of maximum inhibition of the edema of 89.99 ± 4.08. The number of cramps in the mice treated with the mixture at a dose of 150 mg/kg is significantly lower (29.80 ± 1.92) than those of the group treated with Tramadol (42.00 ± 2.70), respectively. The toxicity results show no signs of toxicity with an LD greater than 150 mg/Kg. These interesting results show that the two plants' EOs had an important anti-inflammatory, analgesic, and antioxidant activity, and also a powerful synergistic effect, which encourages further in-depth investigations on their pharmacological proprieties.
天然生物活性分子的研究是当前研究的重点。 和 是摩洛哥人在传统治疗几种与炎症有关的疾病中广泛使用的两种药用植物。本研究旨在评估这两种药用植物精油的单独和联合抗氧化、抗炎和镇痛作用，以及它们的潜在毒性。精油采用 Clevenger 型仪器水蒸馏法提取。抗氧化活性通过两种方法进行评估：DPPH 自由基清除率和铁还原率。通过评估角叉菜胶注射引起的水肿发展来评估抗炎活性，而根据腹腔注射醋酸（0.7%）引起的腹部扭曲次数来评估镇痛能力。进行急性口服毒性试验以评估研究中 EO 的潜在毒性，然后分析血液生化参数。两种抗氧化测试的结果表明，我们的提取物混合物具有良好的铁还原能力和非常有趣的 DPPH 自由基清除能力，其 IC 约为 4.38 ± 0.98 μg/mL，高于基准抗氧化剂 BHT。抗炎试验表明，以 150 mg/kg 的剂量口服给药的混合物具有更好的活性，超过了 1%双氯芬酸钠，水肿最大抑制率为 89.99 ± 4.08%。以 150 mg/kg 剂量治疗的混合物处理的小鼠的痉挛数明显较低（29.80 ± 1.92）与曲马多（42.00 ± 2.70）治疗的组相比。毒性结果显示没有毒性迹象，LD 大于 150 mg/Kg。这些有趣的结果表明，两种植物的精油具有重要的抗炎、镇痛和抗氧化活性，并且具有强大的协同作用，这鼓励进一步深入研究其药理学特性。
