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Native bromoperoxidases do not bind to nitrocellulose: use of DEAE-cellulose as an alternative in blotting.

作者信息

Jordan P, Vilter H

机构信息

Institut für Pharmazeutische Biologie, Universität Bonn, Federal Republic of Germany.

出版信息

Electrophoresis. 1990 Aug;11(8):653-5. doi: 10.1002/elps.1150110814.

DOI:10.1002/elps.1150110814
PMID:2289467
Abstract

Bromoperoxidases were investigated by protein blotting after polyacrylamide gel electrophoresis. While the denatured proteins bound to nitrocellulose, the native enzymes did not. Instead, they could be transferred successfully to DEAE-cellulose. Procedures for immunostaining and glycoprotein detection with concanavalin A on DEAE-cellulose are described. The results indicate that binding of native proteins to nitrocellulose can not necessarily be assumed. DEAE-cellulose is suitable both to investigate this phenomenon or as a substitute for nitrocellulose in blotting of native proteins.

摘要

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