Essén-Gustavsson B, Tesch P A
Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Department of Medicine and Surgery, Uppsala.
Eur J Appl Physiol Occup Physiol. 1990;61(1-2):5-10. doi: 10.1007/BF00236686.
Nine bodybuilders performed heavy-resistance exercise activating the quadriceps femoris muscle. Intermittent 30-s exhaustive exercise bouts comprising 6-12 repetitions were interspersed with 60-s periods for 30 min. Venous blood samples were taken repeatedly during and after exercise for analyses of plasma free fatty acid (FFA) and glycerol concentration. Muscle biopsies were obtained from the vastus lateralis muscle before and after exercise and assayed for glycogen, glycerol-3-phosphate, lactate and triglyceride (TG) content. The activities of citrate synthase (CS), lactate dehydrogenase, hexokinase (HK), myokinase, creatine kinase and 3-hydroxyacyl-CoA dehydrogenase (HAD), were analysed. Histochemical staining procedures were used to assess fibre type composition, fibre area and capillary density. TG content before and after exercise averaged (SD) 23.9 (13.3) and 16.7 (6.4) mmol kg-1 dry wt. The basal triglyceride content varied sixfold among individuals and the higher the levels the greater was the change during exercise. The glycogen content decreased (P less than 0.001) from 690 (82) to 495 (95) mmol kg-1 dry wt. and lactate and glycerol-3-phosphate increased (P less than 0.001) to 79.5 (5.5) and 14.5 (7.3) mmol kg-1 dry wt., respectively, after exercise. The HK and HAD/CS content respectively correlated with glycogen or TG content at rest and with changes in these metabolites during exercise. FFA and glycerol concentrations increased slightly (P less than 0.001) during exercise. Lipolysis may, therefore, provide energy during heavy-resistance exercise of relatively short duration. Also, storage and utilization of intramuscular substrates appear to be influenced by the metabolic profile of muscle.
九名健美运动员进行了激活股四头肌的大重量抗阻训练。间歇性进行30秒的力竭训练，每组包含6 - 12次重复动作，期间穿插60秒的休息时间，共持续30分钟。在运动期间及运动后多次采集静脉血样，用于分析血浆游离脂肪酸（FFA）和甘油浓度。在运动前后从股外侧肌获取肌肉活检样本，检测糖原、3 - 磷酸甘油、乳酸和甘油三酯（TG）含量。分析了柠檬酸合酶（CS）、乳酸脱氢酶、己糖激酶（HK）、肌激酶、肌酸激酶和3 - 羟酰基辅酶A脱氢酶（HAD）的活性。采用组织化学染色方法评估纤维类型组成、纤维面积和毛细血管密度。运动前后TG含量平均（标准差）分别为23.9（13.3）和16.7（6.4）mmol·kg⁻¹干重。个体间基础甘油三酯含量相差六倍，且含量越高，运动期间变化越大。糖原含量从690（82）mmol·kg⁻¹干重降至495（95）mmol·kg⁻¹干重（P < 0.001），运动后乳酸和3 - 磷酸甘油分别增加至79.5（5.5）和14.5（7.3）mmol·kg⁻¹干重（P < 0.001）。HK和HAD/CS含量分别与静息时的糖原或TG含量以及运动期间这些代谢物的变化相关。运动期间FFA和甘油浓度略有升高（P < 0.001）。因此，在相对短时间的大重量抗阻运动中，脂肪分解可能提供能量。此外，肌肉内底物的储存和利用似乎受肌肉代谢特征的影响。
