人支气管肺泡灌洗细胞与鲁米诺依赖性化学发光
Human bronchoalveolar lavage cells and luminol-dependent chemiluminescence.
作者信息
Williams A J, Cole P J
出版信息
J Clin Pathol. 1981 Feb;34(2):167-71. doi: 10.1136/jcp.34.2.167.
Abstract
Human bronchoalveolar lavage cells from several different disease states were examined by the technique of zymosan-stimulated, luminol-dependent chemiluminescence. Light production correlated well with polymorphonuclear leucocyte contamination of the alveolar macrophage suspension but not with lymphocyte contamination. Regression analysis indicated that human alveolar macrophages produce little if any luminol-dependent chemiluminescence. Further investigation of metabolic activity, using measurements of superoxide release, oxygen consumption, and lucigenin-dependent chemiluminescence, showed that "respiratory burst" activity in alveolar macrophages was stimulated by opsonised zymosan.
摘要
采用酵母聚糖刺激、鲁米诺依赖的化学发光技术，对来自几种不同疾病状态的人支气管肺泡灌洗细胞进行了检测。发光与肺泡巨噬细胞悬液中的多形核白细胞污染密切相关，但与淋巴细胞污染无关。回归分析表明，人肺泡巨噬细胞产生的鲁米诺依赖的化学发光极少，甚至没有。通过测量超氧化物释放、耗氧量和光泽精依赖的化学发光对代谢活性进行的进一步研究表明，调理酵母聚糖可刺激肺泡巨噬细胞中的“呼吸爆发”活性。
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