Laser Clinic, Karlsruhe, Germany.
J Dtsch Dermatol Ges. 2012 Dec;10(12):913-8. doi: 10.1111/j.1610-0387.2012.07997.x. Epub 2012 Aug 17.
Laser treatment of onychomycosis is the object of considerable interest. Laser therapy could be a safe and cost-effective treatment modality without the disadvantages of drugs. Some studies have described the inhibitory effects of lasers on the growth of fungal colonies. We therefore examined the effects of various laser wavelengths, which have previously shown inhibitory potential, on the fungal isolate Trichophyton rubrum.
Isolates of fungal colonies were placed clockwise on culture plates. Each culture plate was irradiated on one half with one of the following treatment regimens: 1064 nm-Q-switched Nd:YAG laser at 4 J/cm(2) and 8 J/cm(2) ; 532 nm-Q-switched Nd:YAG laser at 8 J/cm(2) ; 1064 nm-long-pulsed Nd:YAG laser at 45 J/cm(2) or 100 J/cm(2) . The other half remained untreated. Standardized photographs were taken and areas of treated and untreated colonies were compared for growth inhibition.
There was no inhibition of fungal growth in any of the treated plates. Differences in size between treated and untreated colonies were not significant (p > 0.10).
In this in vitro study Nd:YAG laser treatment of Trichophyton rubrum colonies failed to inhibit fungal growth. Nevertheless there might be an effectiveness in vivo which has to be clarified by clinical studies.
激光治疗甲真菌病是一个备受关注的领域。激光治疗可能是一种安全且具有成本效益的治疗方法，没有药物的缺点。一些研究已经描述了激光对真菌菌落生长的抑制作用。因此，我们检查了先前显示出抑制潜力的各种激光波长对真菌分离株红色毛癣菌的影响。
将真菌菌落的分离物按顺时针方向放置在培养板上。每个培养板的一半用以下治疗方案之一进行照射：4 J/cm(2) 和 8 J/cm(2) 的 1064nm-Q 开关 Nd:YAG 激光；8 J/cm(2) 的 532nm-Q 开关 Nd:YAG 激光；45 J/cm(2) 或 100 J/cm(2) 的 1064nm 长脉冲 Nd:YAG 激光。另一半保持未处理。拍摄标准化照片，并比较处理和未处理菌落的区域以抑制生长。
在任何处理的平板中都没有抑制真菌生长。处理和未处理菌落之间的大小差异没有统计学意义（p > 0.10）。
在这项体外研究中，Nd:YAG 激光治疗红色毛癣菌菌落未能抑制真菌生长。然而，在体内可能具有有效性，这需要通过临床研究来阐明。
