Ghavam Seyed Alireza, Aref Saeed, Mohajerani Ezedin, Shidfar Mohammad Reza, Moravvej Hamideh
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Tehran University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2015 Winter;6(1):10-6.
Trichophyton rubrum is one of the most common species of dermatophytes which affects superficial keratinous tissue. It is not especially virulent but it can be responsible for considerable morbidity. Although there are different therapeutic modalities to treat fungal infections, clinicians are searching for alternative treatment because of the various side effects of the present therapeutic methods. As a new procedure, Laser therapy has brought on many advantages in clinical management of dermatophytes. Possible inhibitory potential of laser irradiation on fungal colonies was investigated invitro in this study.
A total of 240 fungal plates of standard size of trichophyton rubrum colonies that had been cultured from the lesions of different patients at the mycology laboratory, were selected. Each fungal plate was assigned as control or experimental group. Experimental plates were irradiated by a laser system (low power laser or different wavelength of high power laser). The effects of different laser wavelengths and energies on isolated colonies were assessed. After laser irradiation, final size of colonies was measured on the first, the 7th and the 14th day after laser irradiation.
Although low power laser irradiation did not have any inhibitory effect on fungal growth, the Q-Switched Neodymium-Doped Yttrium Aluminium Garnet (Nd:YAG) laser 532nm at 8j/cm(2), Q-Switched Nd:YAG laser 1064nm at 4j/cm(2) to 8j/cm(2) and Pulsed dye laser 595nm at 8j/cm(2) to 14j/cm(2) significantly inhibited growth of trichophyton rubrum in vitro.
Q-Switched Nd:YAG 532nm at 8j/cm(2), Q-Switched Nd:YAG laser 1064nm at 4j/cm(2) to 8j/cm(2) and pulsed dye laser (PDL) 595nm at 8j/cm(2) to 14j/cm(2) can be effective to suppress trichophyton rubrum growth.
红色毛癣菌是最常见的皮肤癣菌之一，可侵犯浅表角质组织。它的致病性并不特别强，但可导致相当高的发病率。尽管有多种治疗真菌感染的方法，但由于现有治疗方法存在各种副作用，临床医生仍在寻找替代治疗方法。作为一种新的治疗手段，激光疗法在皮肤癣菌的临床治疗中带来了诸多优势。本研究在体外研究了激光照射对真菌菌落的潜在抑制作用。
从真菌学实验室不同患者皮损处培养出共240个标准大小的红色毛癣菌菌落平板。每个真菌平板分为对照组或实验组。实验组平板用激光系统（低功率激光或不同波长的高功率激光）照射。评估不同激光波长和能量对分离菌落的影响。激光照射后，在照射后的第1天、第7天和第14天测量菌落的最终大小。
尽管低功率激光照射对真菌生长没有任何抑制作用，但波长532nm、能量8j/cm²的调Q掺钕钇铝石榴石（Nd:YAG）激光、波长1064nm、能量4j/cm²至8j/cm²的调Q Nd:YAG激光以及波长595nm、能量8j/cm²至14j/cm²的脉冲染料激光在体外均能显著抑制红色毛癣菌的生长。
波长532nm、能量8j/cm²的调Q Nd:YAG激光、波长1064nm、能量4j/cm²至8j/cm²的调Q Nd:YAG激光以及波长595nm、能量8j/cm²至14j/cm²的脉冲染料激光可有效抑制红色毛癣菌的生长。
