Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland.
J Med Chem. 2012 Sep 27;55(18):7988-97. doi: 10.1021/jm301092r. Epub 2012 Sep 7.
To control the selective adhesion of human endothelial cells and human serum proteins to bioceramics of different compositions, a multifunctional ligand containing a cyclic arginine-glycine-aspartate (RGD) peptide, a tetraethylene glycol spacer, and a gallate moiety was designed, synthesized, and characterized. The binding of this ligand to alumina-based, hydroxyapatite-based, and calcium phosphate-based bioceramics was demonstrated. The conjugation of this ligand to the bioceramics induced a decrease in the nonselective and integrin-selective binding of human serum proteins, whereas the binding and adhesion of human endothelial cells was enhanced, dependent on the particular bioceramics.
为了控制人内皮细胞和人血清蛋白对不同组成的生物陶瓷的选择性黏附，设计、合成并表征了一种多功能配体，该配体含有环状精氨酸-甘氨酸-天冬氨酸（RGD）肽、四乙二醇间隔基和没食子酸部分。证明了该配体与氧化铝基、羟基磷灰石基和磷酸钙基生物陶瓷的结合。该配体与生物陶瓷的偶联导致非选择性和整合素选择性结合的人血清蛋白减少，而人内皮细胞的结合和黏附增强，这取决于特定的生物陶瓷。
