Zhou Wei, Feng Yakai, Yang Jing, Fan Jiaxu, Lv Juan, Zhang Li, Guo Jintang, Ren Xiangkui, Zhang Wencheng
School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Nankai District, Tianjin, 300072, China.
J Mater Sci Mater Med. 2015 Jan;26(1):5386. doi: 10.1007/s10856-015-5386-6. Epub 2015 Jan 20.
Electrospun scaffolds of silk fibroin (SF) and poly(lactide-co-glycolide) (PLGA) were prepared to mimic the morphology and chemistry of the extracellular matrix. The SF/PLGA scaffolds were treated with ethanol to improve their usability. After ethanol treatment the scaffolds exhibited a smooth surface and uniform fibers. SF transformed from random coil conformation to β-sheet structure after ethanol treatment, so that the SF/PLGA scaffolds showed low hydrophilicity and dissolving rate in water. The mechanical properties and the hydrophilicity of the blended fibrous scaffolds were affected by the weight ratio of SF and PLGA. During degradation of ethanol-treated SF/PLGA scaffolds in vitro, the fibers became thin along with the degradation time. Human umbilical vein endothelial cells (HUVECs) were seeded onto the ethanol-treated nanofibrous scaffolds for cell viability, attachment and morphogenesis studies. These SF/PLGA scaffolds could enhance the viability, spreading and attachment of HUVECs. Based on these results, these ethanol-treated scaffolds are proposed to be a good candidate for endothelial cell growth.
制备了丝素蛋白（SF）和聚乳酸-羟基乙酸共聚物（PLGA）的电纺支架，以模拟细胞外基质的形态和化学性质。对SF/PLGA支架进行乙醇处理以提高其适用性。乙醇处理后，支架呈现出光滑的表面和均匀的纤维。乙醇处理后，SF从无规卷曲构象转变为β-折叠结构，使得SF/PLGA支架在水中表现出低亲水性和低溶解速率。共混纤维支架的力学性能和亲水性受SF与PLGA重量比的影响。在体外乙醇处理的SF/PLGA支架降解过程中，纤维随着降解时间而变细。将人脐静脉内皮细胞（HUVECs）接种到乙醇处理的纳米纤维支架上，进行细胞活力、黏附及形态发生研究。这些SF/PLGA支架可增强HUVECs的活力、铺展和黏附。基于这些结果，这些乙醇处理的支架被认为是内皮细胞生长的良好候选材料。
