精子、胚胎和体细胞冷冻保存在猪遗传物质的保存、转移和再衍生中的新兴应用。
Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics.
机构信息
Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA.
出版信息
Theriogenology. 2012 Nov;78(8):1720-9. doi: 10.1016/j.theriogenology.2012.06.003. Epub 2012 Aug 13.
Advances in porcine assisted reproductive technology (ART) make it possible to use cryopreserved sperm, embryos and somatic cells in the maintenance, relocation and regeneration of swine genetics. In this review, development of key application-limiting technology is discussed in each cell type, focusing on the efficiencies, ease of storage and transportation, and minimization of pathogen transmission. Methods to regenerate swine genetics and/or models using frozen sperm, embryos and somatic cells in combination with other porcine ARTs, such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transplantation (SCNT), are also discussed. The applications of these ARTs utilizing cryopreserved cells will greatly increase the efficiency as well as biosecurity for maintenance, relocation and rederivation of swine genetics/models.
猪辅助生殖技术（ART）的进步使得使用冷冻保存的精子、胚胎和体细胞核在猪遗传资源的保存、迁移和再生中成为可能。在本综述中，讨论了每种细胞类型中关键应用限制技术的发展，重点关注效率、储存和运输的便利性，以及最小化病原体传播的方法。还讨论了使用冷冻精子、胚胎和体细胞核，结合其他猪辅助生殖技术（如体外受精（IVF）、胞浆内精子注射（ICSI）和体细胞核移植（SCNT））来再生猪遗传资源和/或模型的方法。这些利用冷冻细胞的 ART 的应用将极大地提高猪遗传资源/模型的保存、迁移和再衍生的效率和生物安全性。
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