Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar--608002, Tamilnadu, India.
Phytother Res. 2013 Jul;27(7):999-1005. doi: 10.1002/ptr.4826. Epub 2012 Aug 17.
Cancer is the second leading cause of death worldwide and is increasing at an alarming rate. The present study was to evaluate the antiproliferative effects of hesperetin, a flavonoid commonly found in many herbal medicines and foods, on aberrant crypt foci (ACF), argyrophylic nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats. Rats were given subcutaneous injections of DMH (20 mg/kg body weight) weekly for 15 weeks to induce carcinogenesis, and hesperetin was administered orally at the dose of 20 mg/kg body weight. DMH exposure alone produced a high incidence of ACF and showed positive staining for PCNA and AgNORs in colonic tissues. Supplementation with hesperetin lowered the PCNA labeling index and suppressed the formation of ACF in the rats with colon cancer. These results clearly reveal that dietary hesperetin possesses antiproliferative ability against chemically induced colon tumourigenesis.
癌症是全球第二大死亡原因，而且其发病率正在以惊人的速度上升。本研究旨在评估橙皮苷（一种在许多草药和食物中常见的类黄酮）对 1,2-二甲基肼（DMH）诱导的大鼠结肠癌中异常隐窝病灶（ACF）、银染核仁组成区（AgNORs）和增殖细胞核抗原（PCNA）的抗增殖作用。大鼠每周接受皮下注射 DMH（20mg/kg 体重），共 15 周，以诱导癌变，同时给予橙皮苷 20mg/kg 体重口服。DMH 暴露本身可导致 ACF 的高发生率，并在结肠组织中显示出 PCNA 和 AgNORs 的阳性染色。补充橙皮苷可降低 PCNA 标记指数，并抑制结肠癌大鼠中 ACF 的形成。这些结果清楚地表明，膳食橙皮苷具有抑制化学诱导的结肠肿瘤发生的抗增殖能力。
