Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Analyst. 2012 Oct 7;137(19):4577-83. doi: 10.1039/c2an35708a.
A new ferrocene derivative (1-[(4-amino) phenylethynyl]ferrocene, Fc-NH(2)) was synthesized for the first time. The ferrocene derivative molecule contained the phenylethynyl skeleton, ferrocene and amino groups with excellent electrochemical properties. The graphene/Fc-NH(2) nanocomposite was prepared by mixing graphene solution and Fc-NH(2) solution in one pot and the nanocomposite was utilized to construct a Nafion/graphene/Fc-NH(2) modified glassy carbon electrode (GCE). The ferrocene derivative immobilized on the graphene can enhance the charge-transport ability of the nanocomposite, stabilize the graphene and prevent the leakage of ferrocene. The detection signal of dopamine (DA) was significantly amplified on the Nafion/graphene/Fc-NH(2)/GCE. It was experimentally demonstrated that the signal enhancement results from the synergy amplification effect of graphene and the Fc-NH(2). The oxidation peak currents of DA were linearly related to the concentrations in the range of 5 × 10(-8) to 2 × 10(-4) M with the detection limit of 20 nM in the absence of uric acid (UA) and ascorbic acid (AA). In the presence of 10(-3) M AA and 10(-4) M UA, the linear response range was 1 × 10(-7) to 4 × 10(-4) M, and the detection limit was 50 nM at S/N = 3. Using the proposed Nafion/Fc-NH(2)/graphene/GCE, DA was successfully determined in real samples with the standard addition method.
一种新型二茂铁衍生物（1-[(4-氨基)苯乙炔基]二茂铁，Fc-NH(2)）被首次合成。该二茂铁衍生物分子含有苯乙炔骨架、二茂铁和氨基，具有优异的电化学性能。通过将氧化石墨烯溶液和 Fc-NH(2)溶液混合一锅法制备了氧化石墨烯/Fc-NH(2)纳米复合材料，并将该纳米复合材料用于构建 Nafion/氧化石墨烯/Fc-NH(2)修饰的玻碳电极（GCE）。固定在氧化石墨烯上的二茂铁衍生物可以增强纳米复合材料的电荷传输能力，稳定氧化石墨烯并防止二茂铁的泄漏。在 Nafion/氧化石墨烯/Fc-NH(2)/GCE 上，多巴胺（DA）的检测信号得到了显著放大。实验证明，这种信号增强是由于氧化石墨烯和 Fc-NH(2)的协同放大效应。在没有尿酸（UA）和抗坏血酸（AA）的情况下，DA 的氧化峰电流在 5×10(-8)到 2×10(-4) M 的浓度范围内呈线性关系，检测限为 20 nM。在 10(-3) M AA 和 10(-4) M UA 的存在下，线性响应范围为 1×10(-7)到 4×10(-4) M，检测限为 50 nM（S/N = 3）。使用所提出的 Nafion/Fc-NH(2)/氧化石墨烯/GCE，采用标准加入法成功测定了实际样品中的 DA。
