Pardhasaradhi B V V, Reddy Madhurima, Ali A Mubarak, Kumari A Leela, Khar Ashok
Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Indian J Biochem Biophys. 2004 Aug;41(4):167-72.
The plant, Annona squamosa traditionally known as custard apple possesses potent bioactive principles in all its parts. The effect of aqueous and organic extracts from defatted seeds of A. squamosa was studied on a rat histiocytic tumour cell line, AK-5. Both the extracts caused significant apoptotic tumour cell death with enhanced caspase-3 activity, down regulation of antiapoptotic genes Bcl-2 and Bcl(XL), and enhanced the generation of intracellular ROS, which correlated well with the decreased levels of intracellular GSH. In addition, DNA fragmentation and annexin-V staining confirmed that the extracts induced apoptosis in tumour cells through the oxidative stress. Aqueous extracts of A. squamosa seeds possessed significant antitumor activity in vivo against AK-5 tumor.
番荔枝，传统上称为释迦果，其各个部位都含有强大的生物活性成分。研究了番荔枝脱脂种子的水提取物和有机提取物对大鼠组织细胞瘤细胞系AK-5的作用。两种提取物均导致肿瘤细胞显著凋亡死亡，伴有半胱天冬酶-3活性增强、抗凋亡基因Bcl-2和Bcl(XL)下调，并增强了细胞内活性氧的生成，这与细胞内谷胱甘肽水平降低密切相关。此外，DNA片段化和膜联蛋白-V染色证实，提取物通过氧化应激诱导肿瘤细胞凋亡。番荔枝种子的水提取物在体内对AK-5肿瘤具有显著的抗肿瘤活性。
